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别名: BSA, 牛血清白蛋白, 白蛋白牛血清
CAS号 9048-46-8
EINECS 编号 232-936-2
MDL number MFCD00130384
| assay | ≥98% |
| mol wt | ~66 kDa |
| purified by | heat shock fractionation |
| origin | Australia origin |
| fatty acid content | <0.05% |
| impurities | ≤0.05% IgG |
| pH | 7 |
| foreign activity | protease ≤0.05 U/mg |
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过
Bovine serum albumin (BSA) is made up of 583 amino acids, with no carbohydrates.[6] It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of BSA becomes reactive flexible on heating.[8]BSA acts as a model protein in various studies, such as immunodiagnostic procedures, cell culture media clinical chemistry. It is also relevant in foods containing bovine milk meat.
Appl- Bovine serum albumin has been used as a supplement of phosphate buffered saline (PBS) to store brain tissue sections.[4] It has also been used as a blocking agent for cells for immunofluorescence analysis.[5][7]
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