2-Plex Phospho/Total Akt1 Beads Mag 20X

Analytes Available	Species Reactivity	Key Applications	Detection Methods
Akt1 (total), Akt1 (Ser473)	H, M, R	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

Akt1 (total), Akt1 (Ser473)

H, M, R

Mplex

Luminex xMAP

Description
Catalogue Number	48-631MAG
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Total/Phospho Akt1 2-Plex Panel
Overview	The MILLIPLEX® MAP Phospho/Total Akt1 2-plex Magnetic Bead Panel (EMD Millipore Catalog # 48-631MAG) has been developed for the simultaneous detection of phosphorylated Akt1 (Ser473) and total Akt1 in a single well using the Luminex system. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples. Each kit has sufficient reagents for one 96 well plate assay. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility. It is possible to multiplex this kit together with other MILLIPLEX® MAP Cell Signaling Phospho/Total 2-plex Magnetic Bead kits or Cell Signaling MAPmate™ kits. For more information please see Section C of “Preparation of Reagents for Immunoassay” in the protocol, available for download on this page. Each MILLIPLEX® MAP cell signaling and cellular metabolism panel/kit includes: •Positive control provided to qualify assay performance •Premixed magnetic beads to capture analytes of interest •Optimized detection antibody cocktails designed to yield consistent analyte profiles within a panel
Background Information	The serine/threonine protein kinase Akt is a key node in the PI3K pathway, one of the primary signaling cascades hyperactivated in human cancer. Akt (also called Protein kinase B or PKB) plays a central role in regulating cell survival, insulin signaling, apoptosis, protein synthesis, cell proliferation, angiogenesis, and tumor formation. Activation of Akt occurs via growth factor signaling, which results in Akt phosphorylation. Activated Akt then phosphorylates a wide range of substrates including transcription factors, kinases and other important signaling proteins. Three isoforms (Akt1, Akt2, Akt3) of Akt exist, and emerging evidence demonstrates that the three Akt isoforms may have unique, isoform-specific roles in key cellular processes. The crucial regulatory phosphorylation sites for the human Akt isoforms are Ser473 and Thr308 for Akt1, Ser474 and Thr309 for Akt2, and Ser472 and Thr305 for Akt3.

Description

Catalogue Number

48-631MAG

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP Total/Phospho Akt1 2-Plex Panel

Overview

The MILLIPLEX® MAP Phospho/Total Akt1 2-plex Magnetic Bead Panel (EMD Millipore Catalog # 48-631MAG) has been developed for the simultaneous detection of phosphorylated Akt1 (Ser473) and total Akt1 in a single well using the Luminex system. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples. Each kit has sufficient reagents for one 96 well plate assay.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility.

It is possible to multiplex this kit together with other MILLIPLEX® MAP Cell Signaling Phospho/Total 2-plex Magnetic Bead kits or Cell Signaling MAPmate™ kits. For more information please see Section C of “Preparation of Reagents for Immunoassay” in the protocol, available for download on this page.

Each MILLIPLEX® MAP cell signaling and cellular metabolism panel/kit includes:
•Positive control provided to qualify assay performance
•Premixed magnetic beads to capture analytes of interest
•Optimized detection antibody cocktails designed to yield consistent analyte profiles within a panel

Background Information

The serine/threonine protein kinase Akt is a key node in the PI3K pathway, one of the primary signaling cascades hyperactivated in human cancer. Akt (also called Protein kinase B or PKB) plays a central role in regulating cell survival, insulin signaling, apoptosis, protein synthesis, cell proliferation, angiogenesis, and tumor formation. Activation of Akt occurs via growth factor signaling, which results in Akt phosphorylation. Activated Akt then phosphorylates a wide range of substrates including transcription factors, kinases and other important signaling proteins. Three isoforms (Akt1, Akt2, Akt3) of Akt exist, and emerging evidence demonstrates that the three Akt isoforms may have unique, isoform-specific roles in key cellular processes. The crucial regulatory phosphorylation sites for the human Akt isoforms are Ser473 and Thr308 for Akt1, Ser474 and Thr309 for Akt2, and Ser472 and Thr305 for Akt3.

Product Information
Detection method	Luminex xMAP
Configuration	Premixed
Panel Type	Cell Signaling

Product Information

Detection method

Luminex xMAP

Configuration

Premixed

Panel Type

Cell Signaling

Applications
Application	Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples.
Key Applications	Multiplexing
Application Notes	• An overnight (4°C) incubation is recommended for best results. • This assay requires 25 µL diluted cell lysate per well. • This kit must be run using Assay Buffer 2 (provided). • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL) Additional Cell Signaling Phospho/Total 2-plex assays or Phospho-MAPmates may be combined with this kit, up to a maximum of 9 additional 2-plex assays or single plex Cell Signaling MAPmates. The following assays should not be plexed with the Phospho/Total Akt1 2-plex Magnetic Bead Panel: Catalog # 48-618MAG, Catalog # 48-632MAG, Catalog # 48-633MAG, Catalog # 46-677MAG, Catalog # 46-675MAG, Total or panTyr MAPmates.

Applications

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples.

Key Applications

Multiplexing

Application Notes

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)

Additional Cell Signaling Phospho/Total 2-plex assays or Phospho-MAPmates may be combined with this kit, up to a maximum of 9 additional 2-plex assays or single plex Cell Signaling MAPmates. The following assays should not be plexed with the Phospho/Total Akt1 2-plex Magnetic Bead Panel: Catalog # 48-618MAG, Catalog # 48-632MAG, Catalog # 48-633MAG, Catalog # 46-677MAG, Catalog # 46-675MAG, Total or panTyr MAPmates.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Human Mouse Rat
Analytes Available	Akt1 (total) Akt1 (Ser473)

Biological Information

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Species Reactivity

Human
Mouse
Rat

Analytes Available

Akt1 (total)
Akt1 (Ser473)

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging Information
Material Size	96-well plate
Material Package	96-well plate

Packaging Information

Material Size

96-well plate

Material Package

96-well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

2-Plex Phospho/Total Akt1 Beads Mag 20X

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