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MHSTCMAG-70K-05 Mouse High Sens MAG

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货号：MHSTCMAG-70K-05

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number MHSTCMAG-70KPMX
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAP Mouse High Sensitivity T Cell Premixed Panel - Immunology Multiplex Assay
Background Information Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection method Luminex xMAP
Configuration Premixed
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel Type MAGNETIC Cytokines/Chemokines
Applications
Application Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes • This is an overnight incubation assay.
• A maximum of 25 μL per well of neat or 1:2 diluted serum or plasma can be used. Tissue culture or other media may also be used.
Biological Information
Specificity Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Mouse
Analytes Available
  • GM-CSF
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • KC/CXCL1
  • LIX
  • MCP-1
  • MIP-2
  • TNF-α
Cross Reactivity There is no detectable cross-reactivity within the panel.
Accuracy
  • 82-102%
Physicochemical Information
Sensitivity
  • 0.26 - 11.14 pg/mL
  • Please see protocol for sensitivity of individual analytes.
Linearity of Dilution Please contact technical service for information
Standard Curve Range
  • GM-CSF: 6.1 – 25,000 pg/mL
  • IFNγ: 0.49 – 2,000 pg/mL
  • IL-1α: 3.91 – 16,000 pg/mL
  • IL-1β: 3.91 – 16,000 pg/mL
  • IL-2: 0.98 – 4,000 pg/mL
  • IL-4: 0.10 – 400 pg/mL
  • IL-5: 0.98 – 4,000 pg/mL
  • IL-6: 0.98 – 4,000 pg/mL
  • IL-7: 0.49 – 2,000 pg/mL
  • IL-10: 1.34 – 5,500 pg/mL
  • IL-12 (p70): 2.93 – 12,000 pg/mL
  • IL-13: 3.42 – 14,000 pg/mL
  • IL-17A: 0.49 – 2,000 pg/mL
  • KC/GROα/CXCL1: 0.73 – 3,000 pg/mL
  • LIX: 9.77 – 40,000 pg/mL
  • MCP-1/CCL2: 3.17 – 13,000 pg/mLL
  • MIP-2/CXCL2: 7.32 – 30,000 pg/mL
  • TNFα: 0.49 – 2,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size 96-well plate
Transport Information
Supplemental Information
Specifications

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