Human HS 384 T Cell MAG Premix 21plxBULK

Analytes Available	Species Reactivity	Key Applications	Detection Methods
Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α, TNF-α	H	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α, TNF-α

Mplex

Luminex xMAP

Description
Catalogue Number	HSTCMAG384PX21BK
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel-Space Saver (Bulk) Packaging
Overview	EMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel is t to be used for the simultaneous quantification of these analytes in serum, plasma, or tissue/cell culture supernatant: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP1alpha/CCL3, MIP1beta/CCL4, MIP-3alpha/CCL20 and TNFalpha. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: • Quality controls (QCs) provided to qualify assay performance • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency • Optimized serum matrix to mimic native analyte environment • Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information	Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases. The 384-well Human High Sensitivity T Cell Panel is the 384-well version of the current 96-well Human High Sensitivity T Cell Panel, and it exceeds expectations in terms of throughput, sensitivity (see table) and sample detectability. Data obtained from the High Sensitivity T Cell Panel will correlate with data for the respective cytokines in the Human Cytokine/Chemokine Panels I, II and III, furthering your ability to measure specific cytokine response in both normal and disease states.

Description

Catalogue Number

HSTCMAG384PX21BK

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel-Space Saver (Bulk) Packaging

Overview

EMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel is t to be used for the simultaneous quantification of these analytes in serum, plasma, or tissue/cell culture supernatant: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP1alpha/CCL3, MIP1beta/CCL4, MIP-3alpha/CCL20 and TNFalpha.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:

• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel

EMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

Background Information

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

The 384-well Human High Sensitivity T Cell Panel is the 384-well version of the current 96-well Human High Sensitivity T Cell Panel, and it exceeds expectations in terms of throughput, sensitivity (see table) and sample detectability. Data obtained from the High Sensitivity T Cell Panel will correlate with data for the respective cytokines in the Human Cytokine/Chemokine Panels I, II and III, furthering your ability to measure specific cytokine response in both normal and disease states.

Product Information
Detection method	Luminex xMAP
Configuration	Premixed
Precision, %	Inter-assay: <20% Intra-assay: <10%
Panel Type	MAGNETIC Cytokines/Chemokines

Product Information

Detection method

Luminex xMAP

Configuration

Premixed

Precision, %

Inter-assay: <20%
Intra-assay: <10%

Panel Type

MAGNETIC Cytokines/Chemokines

Applications
Application	This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay.
Key Applications	Multiplexing
Application Notes	• This is an overnight incubation. • Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. • This kit may be used in serum, plasma or tissue/cell lysate and culture supernatant.

Applications

Application

This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay.

Key Applications

Multiplexing

Application Notes

• This is an overnight incubation.
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
• This kit may be used in serum, plasma or tissue/cell lysate and culture supernatant.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Human
Analytes Available	Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL-10 IL-12 (p70) IL-13 IL-17A IL-21 IL-23 ITAC MIP-1α MIP-1β MIP-3α TNF-α
Cross Reactivity	There is no detectable cross-reactivity within the panel.
Accuracy	79% – 108%

Biological Information

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Species Reactivity

Human

Analytes Available

Fractalkine
GM-CSF
IFNγ
IL-1β
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12 (p70)
IL-13
IL-17A
IL-21
IL-23
ITAC
MIP-1α
MIP-1β
MIP-3α
TNF-α

Cross Reactivity

There is no detectable cross-reactivity within the panel.

Accuracy

79% – 108%

Physicochemical Information
Sensitivity	Please see kit protocol for individual assay sensitivities.
Linearity of Dilution	Please contact Technical Service for information.
Standard Curve Range	Fractalkine: 18.3 – 75,000 pg/mL GM-CSF: 1.22 – 5,000 pg/mL IFNγ: 0.61 – 2,500 pg/mL IL-1β: 0.49 – 2,000 pg/mL IL-2: 0.49 – 2,000 pg/mL IL-4: 1.83 – 7,500 pg/mL IL-5: 0.49 – 2,000 pg/mL IL-6: 0.18 – 750 pg/mL IL-7: 0.37 – 1,500 pg/mL IL-8: 0.31 – 1,250 pg/mL IL-10: 1.46 – 6,000 pg/mL IL-12 (p70): 0.49 – 2,000 pg/mL IL-13: 0.24 – 1,000 pg/mL IL-17A: 0.73 – 3,000 pg/mL IL-21: 0.24 – 1,000 pg/mL IL-23: 7.93 – 32,500 pg/mL ITAC: 1.46 – 6,000 pg/mL MIP-1α: 0.31 – 1,250 pg/mL MIP-1β: 0.92 – 3,750 pg/mL MIP-3α: 0.61 – 2,500 pg/mL TNFα: 0.43 – 1,750 pg/mL

Physicochemical Information

Sensitivity

Please see kit protocol for individual assay sensitivities.

Linearity of Dilution

Please contact Technical Service for information.

Standard Curve Range

Fractalkine: 18.3 – 75,000 pg/mL
GM-CSF: 1.22 – 5,000 pg/mL
IFNγ: 0.61 – 2,500 pg/mL
IL-1β: 0.49 – 2,000 pg/mL
IL-2: 0.49 – 2,000 pg/mL
IL-4: 1.83 – 7,500 pg/mL
IL-5: 0.49 – 2,000 pg/mL
IL-6: 0.18 – 750 pg/mL
IL-7: 0.37 – 1,500 pg/mL
IL-8: 0.31 – 1,250 pg/mL
IL-10: 1.46 – 6,000 pg/mL
IL-12 (p70): 0.49 – 2,000 pg/mL
IL-13: 0.24 – 1,000 pg/mL
IL-17A: 0.73 – 3,000 pg/mL
IL-21: 0.24 – 1,000 pg/mL
IL-23: 7.93 – 32,500 pg/mL
ITAC: 1.46 – 6,000 pg/mL
MIP-1α: 0.31 – 1,250 pg/mL
MIP-1β: 0.92 – 3,750 pg/mL
MIP-3α: 0.61 – 2,500 pg/mL
TNFα: 0.43 – 1,750 pg/mL

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Storage and Shipping Information

Storage Conditions

Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	384-well plate

Packaging Information

Material Size

384-well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Human HS 384 T Cell MAG Premix 21plxBULK

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