Milliplex Catalog ID.RCI1MAG-87K-05.Rat

Analytes Available	Species Reactivity	Key Applications	Detection Methods
Troponin I (TnI), Troponin T (TnT), Creatine Kinase, Muscle (CKM), FABP3, Follistatin-like Protein 1 (FSTL1), Myosin Light Chain 3 (MYL3), TIMP-1	R	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

Troponin I (TnI), Troponin T (TnT), Creatine Kinase, Muscle (CKM), FABP3, Follistatin-like Protein 1 (FSTL1), Myosin Light Chain 3 (MYL3), TIMP-1

Mplex

Luminex xMAP

Description
Catalogue Number	RCI1MAG-87K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay
Overview	The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 (RCI1MAG-87K) contains all the components necessary to simultaneous quantify any or all of the following 7 analytes in serum and plasma samples: • Cardiac Troponin I (cTnI) • Cardiac Troponin T (cTnT) • Creatine Kinase, Muscle (CKM) • Fatty Acid Binding Protein 3 (FABP3) • Follistatin-like Protein 1 (FSTL1) • Myosin Light Chain 3 (MYL3) • Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) This kit uses a 96-well format, contains lyophilized standard cocktail and two quality controls and can measure up to 38 serum or plasma samples in duplicate.
Background Information	Drug-induced cardiotoxicity can be life threatening and necessitates limiting an otherwise-effective treatment. In fact, cardiovascular toxicity has been reported to account for over 40% of drug candidate withdrawal in premarketing clinical trials. Many therapeutic agents have been reported to induce or exacerbate heart failure including anti-tumor medications, immunomodulating drugs, antidepressants and calcium channel blocking agents to name a few. Conventional biomarkers of cardiotoxicity such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) have gradually been withdrawn due to low tissue specificity. The number of cardiac-specific biomarkers adapted from clinical studies of cardiotoxicity continues to rise. The reverse translational application of these auspicious biomarkers in experimental animal models could improve the power of detection for diagnosis, helping to predict the occurrence of cardiotoxicity in humans. Emerging biomarkers of cardiac injury include multiple categories of proteins: characteristic muscle cell proteins (cTnI, cTnT, FABP3 and MYL3), cytosolic kinase (CKM), secreted heparin-binding protein (FSTL1) and metalloprotease inhibitor (TIMP1). Numerous studies have demonstrated elevated levels of these biomarkers in experimental animal blood samples. In order to meet the increasing need to provide screening assays for experimental biomarkers of drug-induced cardiac injury in pre-clinical and translational research models, EMD Millipore has developed the MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1. This confugurable 7-plex panel is the most versatile system available for cardiac injury screening in pre-clinical and translational research models.

Description

Catalogue Number

RCI1MAG-87K

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay

Overview

The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 (RCI1MAG-87K) contains all the components necessary to simultaneous quantify any or all of the following 7 analytes in serum and plasma samples:

• Cardiac Troponin I (cTnI)
• Cardiac Troponin T (cTnT)
• Creatine Kinase, Muscle (CKM)
• Fatty Acid Binding Protein 3 (FABP3)
• Follistatin-like Protein 1 (FSTL1)
• Myosin Light Chain 3 (MYL3)
• Tissue Inhibitor of Metalloproteinase-1 (TIMP-1)

This kit uses a 96-well format, contains lyophilized standard cocktail and two quality controls and can measure up to 38 serum or plasma samples in duplicate.

Background Information

Drug-induced cardiotoxicity can be life threatening and necessitates limiting an otherwise-effective treatment. In fact, cardiovascular toxicity has been reported to account for over 40% of drug candidate withdrawal in premarketing clinical trials.

Many therapeutic agents have been reported to induce or exacerbate heart failure including anti-tumor medications, immunomodulating drugs, antidepressants and calcium channel blocking agents to name a few. Conventional biomarkers of cardiotoxicity such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) have gradually been withdrawn due to low tissue specificity. The number of cardiac-specific biomarkers adapted from clinical studies of cardiotoxicity continues to rise. The reverse translational application of these auspicious biomarkers in experimental animal models could improve the power of detection for diagnosis, helping to predict the occurrence of cardiotoxicity in humans.

Emerging biomarkers of cardiac injury include multiple categories of proteins: characteristic muscle cell proteins (cTnI, cTnT, FABP3 and MYL3), cytosolic kinase (CKM), secreted heparin-binding protein (FSTL1) and metalloprotease inhibitor (TIMP1). Numerous studies have demonstrated elevated levels of these biomarkers in experimental animal blood samples. In order to meet the increasing need to provide screening assays for experimental biomarkers of drug-induced cardiac injury in pre-clinical and translational research models, EMD Millipore has developed the MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1. This confugurable 7-plex panel is the most versatile system available for cardiac injury screening in pre-clinical and translational research models.

Product Information
Components	Rat Cardiac Injury Standard, 1 vial, lyophilized Rat Cardiac Injury Quality Control 1, 1 vial, lyophilized Rat Cardiac Injury Quality Control 2, 1 vial, lyophilized Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized Assay Buffer, 1 bottle, 30 mL Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea Rat Cardiac Injury Panel 1 Detection Antibodies, 1 bottle, 3.2 mL Rat Cardiac Injury Panel 1 Antibody-Immobilized Beads (20X), 200 µL ea Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL Mixing Bottle, 1 bottle Sample Plate, 96 well format, 1 plate 2 Foil Plate Sealers
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Inter-assay: <15 Intra-assay: <10
Panel Type	MAGNETIC Toxicity

Product Information

Components

Rat Cardiac Injury Standard, 1 vial, lyophilized
Rat Cardiac Injury Quality Control 1, 1 vial, lyophilized
Rat Cardiac Injury Quality Control 2, 1 vial, lyophilized
Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Cardiac Injury Panel 1 Detection Antibodies, 1 bottle, 3.2 mL
Rat Cardiac Injury Panel 1 Antibody-Immobilized Beads (20X), 200 µL ea
Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers

Detection method

Luminex xMAP

Configuration

Design your multiplex kit by choosing available analytes within this panel.

Precision, %

Inter-assay: <15
Intra-assay: <10

Panel Type

MAGNETIC Toxicity

Applications
Application	The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 is a configurable 7-plex kit for the quantification of cTnI, cTnT, CKM (MM-CK), FABP3, FSTL1, MYL3, and TIMP-1 in serum or plasma samples on the Luminex xMAP® instrument platform.
Key Applications	Multiplexing
Application Notes	• This assay requires 25 µL of 1:5 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions). • This assay protocol includes instruction for 2 Hour or Overnight incubation.

Applications

Application

The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 is a configurable 7-plex kit for the quantification of cTnI, cTnT, CKM (MM-CK), FABP3, FSTL1, MYL3, and TIMP-1 in serum or plasma samples on the Luminex xMAP® instrument platform.

Key Applications

Multiplexing

Application Notes

• This assay requires 25 µL of 1:5 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This assay protocol includes instruction for 2 Hour or Overnight incubation.

Biological Information
Specificity	There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity	Rat
Analytes Available	Troponin I (TnI) Troponin T (TnT) Creatine Kinase, Muscle (CKM) FABP3 Follistatin-like Protein 1 (FSTL1) Myosin Light Chain 3 (MYL3) TIMP-1
Assay Panel	Everything you need in a single kit.
Cross Reactivity	There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy	2 Hour Protocol: 80% - 100% Recovery Overnight Protocol: 86% - 103% Recovery Please refer to Assay Protocol for individual analyte accuracy.

Biological Information

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Species Reactivity

Analytes Available

Troponin I (TnI)
Troponin T (TnT)
Creatine Kinase, Muscle (CKM)
FABP3
Follistatin-like Protein 1 (FSTL1)
Myosin Light Chain 3 (MYL3)
TIMP-1

Assay Panel

Everything you need in a single kit.

Cross Reactivity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Accuracy

2 Hour Protocol: 80% - 100% Recovery
Overnight Protocol: 86% - 103% Recovery
Please refer to Assay Protocol for individual analyte accuracy.

Physicochemical Information
Sensitivity	2 Hour Protocol: 9 - 86 pg/mL (MinDC+2SD). Overnight Protocol: 7 - 155 pg/mL (MinDC+2SD). Please see kit protocol for assay sensitivities of individual analytes.
Linearity of Dilution	Contact technical support for this information.
Standard Curve Range	cTnI: 123 – 90,000 pg/mL cTnT: 82 – 60,000 pg/mL CKM: 21 – 15,000 pg/mL FABP3: 41 – 30,000 pg/mL FSTL1: 82 – 60,000 pg/mL MYL3: 4 – 3,000 pg/mL TIMP-1: 41 – 30,000 pg/mL

Physicochemical Information

Sensitivity

2 Hour Protocol: 9 - 86 pg/mL (MinDC+2SD).
Overnight Protocol: 7 - 155 pg/mL (MinDC+2SD).
Please see kit protocol for assay sensitivities of individual analytes.

Linearity of Dilution

Contact technical support for this information.

Standard Curve Range

cTnI: 123 – 90,000 pg/mL
cTnT: 82 – 60,000 pg/mL
CKM: 21 – 15,000 pg/mL
FABP3: 41 – 30,000 pg/mL
FSTL1: 82 – 60,000 pg/mL
MYL3: 4 – 3,000 pg/mL
TIMP-1: 41 – 30,000 pg/mL

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Storage and Shipping Information

Storage Conditions

Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Packaging Information

Material Size

96-well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Milliplex Catalog ID.RCI1MAG-87K-05.Rat

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