NHP Cyto Panel 2 MAG Premix 25 plx Kit

Analytes Available	Species Reactivity	Key Applications	Detection Methods
sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA	NhP	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA

NhP

Mplex

Luminex xMAP

Description
Catalogue Number	PRCYTMAG40K-PX25
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay
Overview	To identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include: • More flexible plate and plate washer options • Improved performance with turbid serum/plasma samples • Assay results equivalent to non-magnetic beads • Automated magnetic washing avoids many problems associated with vacuum filtration washing Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel 2 enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.
Background Information	Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

Description

Catalogue Number

PRCYTMAG40K-PX25

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay

Overview

To identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel 2 enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.

Background Information

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

Product Information
Components	Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized Sample Plate, 96 well format, 1 plate 2 Foil plate sealers Assay Buffer, 2 bottles, 30 mL Bead Diluent, 1 bottle, 3.5 mL Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL Premixed 24-plex Beads, 1 bottle, 3.5 mL RANTES beads, 1 vial
Detection method	Luminex xMAP
Configuration	Premixed
Precision, %	Inter-assay: <15 Intra-assay: <10
Panel Type	MAGNETIC Cytokines/Chemokines

Product Information

Components

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized
Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized
Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized
Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized
Sample Plate, 96 well format, 1 plate
2 Foil plate sealers
Assay Buffer, 2 bottles, 30 mL
Bead Diluent, 1 bottle, 3.5 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL
Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
Premixed 24-plex Beads, 1 bottle, 3.5 mL
RANTES beads, 1 vial

Detection method

Luminex xMAP

Configuration

Premixed

Precision, %

Inter-assay: <15
Intra-assay: <10

Panel Type

MAGNETIC Cytokines/Chemokines

Applications
Application	Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • This assay requires 25 µl of 1:2 diluted plasma or serum or 25 μL cell culture supernatant per well. • RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs.

Applications

Application

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

Key Applications

Multiplexing

Application Notes

• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of 1:2 diluted plasma or serum or 25 μL cell culture supernatant per well.
• RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Non-human Primate
Analytes Available	sCD137 Eotaxin/CCL11 sFasL FGF-2 Fractalkine/CX3CL1 Granzyme A Granzyme B IL-1α IL-2 IL-4 IL-6 IL-16 IL-17A IL-17E/IL-25 IL-21 IL-22 IL-23 IL-28A IL-31 IL-33 IP-10/CXCL10 MIP-3α/CCL20 Perforin RANTES/CCL5 TNFβ/LTA
Cross Reactivity	This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.

Biological Information

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Species Reactivity

Non-human Primate

Analytes Available

sCD137
Eotaxin/CCL11
sFasL
FGF-2
Fractalkine/CX3CL1
Granzyme A
Granzyme B
IL-1α
IL-2
IL-4
IL-6
IL-16
IL-17A
IL-17E/IL-25
IL-21
IL-22
IL-23
IL-28A
IL-31
IL-33
IP-10/CXCL10
MIP-3α/CCL20
Perforin
RANTES/CCL5
TNFβ/LTA

Cross Reactivity

This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range	RANTES/CCL5 (tissue culture): 1 – 750 pg/mL IL-17A: 1 – 1,500 pg/mL Eotaxin/CCL11, IL-6: 3 – 3,000 pg/mL Granzyme B: 4 – 4,000 pg/mL MIP-3α/CCL20: 7 – 7,000 pg/mL sFasL: 8 – 8,000 pg/mL sCD137, IL-2, IP-10/CXCL10: 10 – 10,000 pg/mL IL-21, IL-31: 15 – 15,000 pg/mL FGF-2, IL-33: 20 – 20,000 pg/mL IL-23: 24 – 25,000 pg/mL IL-1α, IL-28A: 29 – 30,000 pg/mL IL-16: 73 – 75,000 pg/mL Granzyme A: 78 – 80,000 pg/mL Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA: 98 – 100,000 pg/mL IL-4: 146 – 150,000 pg/mL IL-17E/IL-25: 293 – 300,000 pg/mL

Physicochemical Information

Sensitivity

Refer to kit protocol for sensitivities of individual biomarkers.

Standard Curve Range

RANTES/CCL5 (tissue culture): 1 – 750 pg/mL
IL-17A: 1 – 1,500 pg/mL
Eotaxin/CCL11, IL-6: 3 – 3,000 pg/mL
Granzyme B: 4 – 4,000 pg/mL
MIP-3α/CCL20: 7 – 7,000 pg/mL
sFasL: 8 – 8,000 pg/mL
sCD137, IL-2, IP-10/CXCL10: 10 – 10,000 pg/mL
IL-21, IL-31: 15 – 15,000 pg/mL
FGF-2, IL-33: 20 – 20,000 pg/mL
IL-23: 24 – 25,000 pg/mL
IL-1α, IL-28A: 29 – 30,000 pg/mL
IL-16: 73 – 75,000 pg/mL
Granzyme A: 78 – 80,000 pg/mL
Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA: 98 – 100,000 pg/mL
IL-4: 146 – 150,000 pg/mL
IL-17E/IL-25: 293 – 300,000 pg/mL

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Storage and Shipping Information

Storage Conditions

Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Packaging Information

Material Size

96-well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

NHP Cyto Panel 2 MAG Premix 25 plx Kit

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