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MilliplexCatalogID.CKT1MAG-97K-06.Canine

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货号：CKT1MAG-97K-06

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Clusterin, Cystatin C, KIM-1, IL-8, Lipocalin-2/NGAL, MCP-1, Osteopontin Ca Mplex Luminex xMAP
Description
Catalogue Number CKT1MAG-97K
Trade Name
  • MILLIPLEX
Description MILLIPLEX® MAP Canine Kidney Toxicity Expanded Magnetic Bead Panel 1, CKT1MAG-97K
Overview The MILLIPLEX® MAP Canine Kidney Toxicity Expanded Magnetic Bead Panel 1, contains all the components necessary to measure the following seven biomarkers in any combination using Luminex® xMAP® technology: Clusterin, Cystatin C, Kidney Injury Molecule-1 (KIM-1), IL-8, Lipocalin-2/NGAL, MCP-1, Osteopontin (OPN). The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate.
Background Information As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers.

In pharmaceutical and chemical industries, the kidney is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.

EMD Millipore provides an expanded valuable research assay to investigate multiple biomarkers of kidney injury in canine urine samples using the Luminex xMAP® instrument platform. The new MILLIPLEX® MAP Canine Kidney Toxicity Expanded Magnetic Bead Panel 1 not only contains the three important biomarkers from the original Canine Kidney Toxicity Panel 1 (Clusterin, Cystatin C and KIM-1), but it also includes specific and sensitive assays for 4 more important nephrotoxicity biomarkers.
References
Product Information
Detection method Luminex xMAP
Configuration Design your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-Assay: < 10
Panel Type MAGNETIC Toxicity
Applications
Application The analytes available for this multiplex kit are: Clusterin, Cystatin C, Kidney Injury Molecule-1 (KIM-1), IL-8, Lipocalin-2/NGAL, MCP-1, Osteopontin (OPN).
Key Applications
  • Multiplexing
Application Notes • Customers should determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• This assay requires 25 µL of 1:2 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This is a 2 hour assay.
Biological Information
Specificity There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Canine
Analytes Available
  • Clusterin
  • Cystatin C
  • KIM-1
  • IL-8
  • Lipocalin-2/NGAL
  • MCP-1
  • Osteopontin
Assay Panel Everything you need in a single kit.
Cross Reactivity There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 89% - 107%
Physicochemical Information
Sensitivity
  • KIM-1: 0.670 pg/mL (MinDC +2SD)
  • All other analytes: 0.005- 0.630 ng/mL (MinDC +2SD)
  • See kit protocol for individual analytes sensitivities.
Linearity of Dilution Contact technical support for this information
Standard Curve Range
  • KIM-1: 1.0 – 700 pg/mL
  • Clusterin: 1.4 – 1,000 ng/mL
  • Cystatin C: 0.01 – 10 ng/mL
  • IL-8: 0.07 – 50 ng/mL
  • Lipocalin-2/NGAL: 0.03 – 20 ng/mL
  • MCP-1: 0.07 – 50 ng/mL
  • Osteopontin: 0.01 – 10 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size 96-well plate
Transport Information
Supplemental Information
Specifications

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