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2-Plex Phospho/Total p38 MAG Panel

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货号：48-624MAG

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
p38 (Total), p38 (Thr180/Tyr182) H, M, R Mplex Luminex xMAP
Description
Catalogue Number 48-624MAG
Trade Name
  • MILLIPLEX
Description MILLIPLEX® MAP 2-Plex Phospho/Total p38 - Cell Signaling Multiplex Assay
Overview The MILLIPLEX® MAP 2-Plex Phospho/Total p38 magnetic bead kit has been developed for the simultaneous detection of phosphorylated p38 (Thr180/Thr182) and total p38 in a single well using the Luminex® system. This detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
Background Information Protein phosphorylation represents a key mechanism used in regulating cellular functions in all eukaryotic cells. Aberrant phosphorylation has been implicated in the onset and development of many diseases including metabolic disorders, inflammatory disease, cancer, etc. Changes in protein phosphorylation can be attributed to changes in both phosphorylation events, as well as changes due to total protein levels. To distinguish the changes in phosphorylation from changes in protein expression it is important to normalize the signal from phosphorylation over the signal from total protein. For this need, the MILLIPLEX® MAP 2-plex Phospho/Total p38 kit has been developed for the simultaneous detection of phosphorylated p38 (Thr180/Tyr182) and total p38 in a single well using the Luminex system. According to the original vendor’s antibody information sheets, phospho-p38 antibody pair detects p38α, p38β and p38γ but not p38-δ, while the total p38 antibody pair detects only p38α. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples. Each kit has sufficient reagents for one 96 well plate assay.
References
Product Information
Components
  • MILLIPLEX® MAP 2-plex Phospho/Total p38, Magnetic Beads (20X), 1 tube, 180 uL
  • MILLIPLEX® MAP 2-plex Phospho/Total p38, Biotin (20X) Detection Antibody, 1 tube, 180 uL
  • MILLIPLEX® MAP Lysis Buffer (1X), 1 bottle, 55 mL
  • MILLIPLEX® MAP Assay Buffer 2 (1X), 1 bottle, 55 mL
  • MILLIPLEX® MAP HeLa Cell Lysate: Unstimulated, 1 vial
  • MILLIPLEX® MAP HeLa Cell Lysate: TNFa/Calyculin A, 1 vial
  • MILLIPLEX® MAP Streptavidin-Phycoerythrin (25X), 1 tube, 150 uL
  • MILLIPLEX® MAP Amplification Buffer (1X), 1 bottle, 3 mL
  • Empty mixing bottles, 3 bottles
  • Sample plates, 96 well format, 2 plates with 4 foil plate sealers
Detection method Luminex xMAP
Configuration Premixed
Panel Type Cell Signaling
Applications
Application Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
Key Applications
  • Multiplexing
Application Notes • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted, filtered cell lysate per well.
• This kit must be run using the provided Assay Buffer.
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL)
Biological Information
Specificity Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • p38 (Total)
  • p38 (Thr180/Tyr182)
Assay Panel Everything you need in a single kit.
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size 96-well plate
Material Package 96-well plate
Transport Information
Supplemental Information
Specifications

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