ChIRP Human HOTAIR lncRNA Probe Set - 炼石商城

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ChIRP Human HOTAIR lncRNA Probe Set

市场价：: 0.00

促销价：: ¥0.00

货号：03-312

品牌：Millipore 密理博

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Replacement Information

Description
Catalogue Number	03-312
Brand Family	Chemicon®
Trade Name	Magna ChIRP
Description	Magna ChIRP Human HOTAIR lncRNA Probe Set
Overview	ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target for biotinylated antisense probes. The Magna ChIRP Human HOTAIR lncRNA Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of Human lncRNA HOTAIR. All probes are biotin-TEG-labeled at the 3-prime end. The oligonucleotides are split to two pools (even and odd) and are supplied ready for use in the ChIRP method.
Background Information	Pervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation. Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP™ RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.

References

Product Information
Presentation	10 mM Tris-HCl, 1mM EDTA, pH 8.0

Applications
Application	The Magna ChIRP Human HOTAIR lncRNA Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of Human lncRNA HOTAIR.
Key Applications	DNA-binding Proteins DNA Sequencing Protein Determination Protein Interaction Assays Purification with magnetic beads

Biological Information
Concentration	Please refer to lot specific datasheet.

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Safety Information according to GHS

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Product Usage Statements
Quality Assurance	Verified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Upon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.

Packaging Information
Material Size	12 reactions/Pk

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