Cu/Zn Superoxide Dismutase ELISA Kit

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Cu/Zn Superoxide Dismutase ELISA Kit

市场价：: 0.00

促销价：: ¥0.00

货号：QIA97-1KIT

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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工作时间

周一至周五：9:00-18:00

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0771-3293894

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Replacement Information

Description
Overview	A colorimetric sandwich ELISA method for detecting Cu/Zn superoxide dismutase (SOD) in different types of biological samples.
Catalogue Number	QIA97
Brand Family	Calbiochem®
Application Data	Recombinant Cu/Zn SOD was diluted in serial two-fold steps in PBS. The mean of three parallel titrations are plotted. Data represents the mean Cu/Zn SOD concentration and the coefficient of variation for each sample. The overall intra-assay coefficient of variation was determined to be 5.1%. Data represents the mean Cu/Zn SOD concentration and the coefficient of variation calculated on 18 determinations of each sample. The overall inter-assay coefficient of variation was determined to be 5.8%. Percent recoveries of expected values.
Materials Required but Not Delivered	• 5 and 10 ml graduated pipettes • 5 to 1000 µl adjustable single channel micropipettes with disposable tips • 50 to 300 µl adjustable multichannel micropipette with disposable tips • Multichannel micropipette reservoir • Beakers, flasks, and cylinders necessary for preparation of reagents • Multichannel wash bottle or automatic wash system for delivery of wash solution • Plate reader capable of reading in 96-well plates at 450 nm with 620 nm as optional reference wavelength • Glass-distilled or deionized water • Calculator with program to perform linear regression analysis

References
References	Herouart, D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3108. Niwa, Y., et al. 1993. Am. J. Pathol. 143, 312. Suzuki, H., et al. 1993. J. Clin. Invest. 91, 2727. Holzgreve, W., et al. 1991. Diagnose Labor. 41, 162. Porstmann, T., et al. 1991. Prenat. Diagn. 11, 195. Shull, S., et al. 1991. J. Biol. Chem. 266, 24398. Porstmann, T., et al. 1990. Hum. Genet. 85, 362. Porstmann, T., et al. 1988. Clin. Chim. Acta 171, 1. Jolly, S.R., et al. 1984. Circ. Res. 54, 277. Goebel, K.M. and Storck, U. 1983. Am. J. Med. 74, 124. Barra, D., et al. 1980. FEBS Lett. 120, 53. Sinet, P.M., et al. 1976. Exp. Cell Res. 97, 47. Harta, J.W. and Deutsch, H.F. 1972. Biol. Chem. 247, 7043.

References

Herouart, D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3108.
Niwa, Y., et al. 1993. Am. J. Pathol. 143, 312.
Suzuki, H., et al. 1993. J. Clin. Invest. 91, 2727.
Holzgreve, W., et al. 1991. Diagnose Labor. 41, 162.
Porstmann, T., et al. 1991. Prenat. Diagn. 11, 195.
Shull, S., et al. 1991. J. Biol. Chem. 266, 24398.
Porstmann, T., et al. 1990. Hum. Genet. 85, 362.
Porstmann, T., et al. 1988. Clin. Chim. Acta 171, 1.
Jolly, S.R., et al. 1984. Circ. Res. 54, 277.
Goebel, K.M. and Storck, U. 1983. Am. J. Med. 74, 124.
Barra, D., et al. 1980. FEBS Lett. 120, 53.
Sinet, P.M., et al. 1976. Exp. Cell Res. 97, 47.
Harta, J.W. and Deutsch, H.F. 1972. Biol. Chem. 247, 7043.

Product Information
Detection method	Colorimetric
Form	96 Tests
Format	96-well plate
Kit contains	Pre-Coated 96-Well Plate, Anti-Cu/Zn SOD/HRP-Conjugate, Cu/Zn SOD Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Phosphate Buffered Saline, Substrate Solution, Stop Solution, Plate Covers, and a user protocol.

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay

Biological Information
Assay time	1.5 h
Sample Type	Cell culture supernatant, serum, plasma, urine, amniotic fluid, and other biological fluids
Species Reactivity	Human

Physicochemical Information
Sensitivity	40 pg/ml

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26-36-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

Product Usage Statements
Intended use	The Calbiochem^® Cu/Zn Superoxide Dismutase ELISA Kit is designed to measure Cu/Zn superoxide dismutase (SOD) in cell culture supernatants and biological fluids like human serum, plasma, urine, and amniotic fluid.

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	+2°C to +8°C
Storage Conditions	Upon arrival store the entire contents of the kit at 4°C.
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information
Kit contains	Pre-Coated 96-Well Plate, Anti-Cu/Zn SOD/HRP-Conjugate, Cu/Zn SOD Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Phosphate Buffered Saline, Substrate Solution, Stop Solution, Plate Covers, and a user protocol.