Assay kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. Translocation of cytochrome c from the mitochondrial fraction to the cytosol is monitored by immunoblotting with the cytochrome c antibody provided with the kit.
Catalogue Number
QIA87
Brand Family
Calbiochem®
Materials Required but Not Delivered
• DMSO • PBS
References
Product Information
Form
100 Tests
Format
Immunoblot
Kit contains
Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, Cytochrome c Antibody, and a user protocol.
Applications
Key Applications
Immunoblotting (Western Blotting)
Biological Information
Assay time
4 h
Sample Type
Cell Extracts
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 32-23/24/25-34-36/37/38-41
Contact with acids liberates very toxic gas. Toxic by inhalation, in contact with skin and if swallowed. Causes burns. Irritating to eyes, respiratory system and skin. Risk of serious damage to eyes.
S Phrase
S: 23-26-36/37/39-45
Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended use
The Cytochrome c Release Apoptosis Assay Kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol that can be used to detect cytochrome c by Western blotting.
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
-20°C
Storage Conditions
Upon arrival store the entire contents of the kit at -20°C. Once opened, store the Mitochondria Extraction Buffer and Cytosol Extraction Buffer at 4°C and the remaining components at -20°C.
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, Cytochrome c Antibody, and a user protocol.
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