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Qiagen 凯杰 Biosharp Omega

首页细胞生物学检测Millipore 密理博>

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Live/Dead Double Staining Kit

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号:QIA76-100TEST

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Detection Methods
Fluorescence
Description
Overview This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test.
Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator.
Catalogue Number QIA76
Brand Family Calbiochem®
Materials Required but Not Delivered Fluorescent microscope with a band-pass filter that detects FITC and rhodamine
Microscope slides and cover slips
References
References Luther, E., and Kamenstsky, L.A. 1996. Cytometry 23, 272.
Frey. T., et al. 1995. Cytometry 21, 265.
Product Information
Detection method Fluorescence
Form 100 Tests
Format Fluorescence microscopy
Kit contains Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
Applications
Biological Information
Assay time 0.5 h
Sample Type Cell suspensions or adherent cells
Species Reactivity
  • A Broad Range Of Species
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase R: 36/37/38-46

Irritating to eyes, respiratory system and skin.
May cause heritable genetic damage.
S Phrase S: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended use The Calbiochem® Live/Dead Double Staining Kit can be used to distinguish between live and dead cells, which is essential for the study of growth control and cell death.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Storage Conditions Upon arrival, store the entire contents of the kit at -20°C. Following initial use of the Staining Buffer, refrigerate (4°C) the remaining buffer.
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
Specifications

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