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Rapid Cell Proliferation Kit

市场价：: 0.00

促销价：: ¥0.00

货号：QIA127-1KIT

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Detection Methods
Colorimetric

Description
Overview	Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
Catalogue Number	QIA127
Brand Family	Calbiochem®
Materials Required but Not Delivered	• Pipettors with disposable tips • Tissue culture grade, flat-bottom 96-well plate • Spectrophotometer capable of measuring absorbance in 96-well plates at a wavelength of 440-460 nm

References
References	Ishiyama, M., et al. 1995. In Vitro Toxicology 8, 187. Liu, S.O., et al 1995. Nat. Med. 1, 267. Ishiyama, M., et al. 1993. Chem. Pharm. Bull. 41, 1118.

Product Information
Detection method	Colorimetric
Form	500 Tests
Format	96-well plate
Kit contains	WST-1, 1-Methoxy PMS, and a user protocol.

Applications

Biological Information
Assay range	1000 - 50,000 Cells
Assay time	0.5 - 4 h
Sample Type	Cultured cells

Physicochemical Information
Sensitivity	1000 cells (adherent); 2500 cells (nonadherent)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Intended use	The Calbiochem^® Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	-20°C
Storage Conditions	Upon arrival store the entire contents of the kit at -20°C.
Protect from Light	Protect from light
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information
Kit contains	WST-1, 1-Methoxy PMS, and a user protocol.

Specifications

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