Calpains are Ca2+-dependent proteinases, which are involved in normal cellular processes including cell proliferation, apoptosis, differentiation, and cell migration. Requires a fluorimeter or microplate reader capable of measuring fluorescence at wavelengths of Excitation max: ~360 nm and Emission max: ~460 nm.
Catalogue Number
QIA120
Brand Family
Calbiochem®
Application Data
Typical standard curve generated by using dilutions of the AMC Standard as indicated in the Detailed Protocol.
Materials Required but Not Delivered
• Pipetman or multi-channel pipetman: use only pipettes that have been carefully calibrated to their target volume • Microplate reader capable of measuring fluorescence at an excitation wavelength of ~360-380 nm and an emission wavelength of ~440-460 nm • Ice bucket to keep reagents cold until use • Phosphate-buffered saline (PBS) for preparation of cell lysates
References
References
Dainese, E., et al. 2002. J. Biol. Chem.277, 40296. Glading, A., et al. 2000. J. Biol. Chem.275, 23908. Ishikara, I., et al. 2000. Neurosci. Lett.279, 97. Nakagawa, T. and Yuan, J. 2000. J. Cell Biol.150, 887. Pariat, M., et al. 2000. Biochem. J.345, 129. Pink, J.J., et al. 2000.Exp. Cell Res.255, 144. Wang, K.K. 2000. Trends Neurosci.23, 20. Reddy, R.K., et al. 1999. J. Biol. Chem.274, 28476. Leist, M., et al. 1998. Mol. Pharmacol.54, 789. Melloni, E., et al. 1998. J. Biol. Chem.273, 12827. Villa, P.G., et al. 1998. J. Cell Sci.111, 713. Wood, D.E., et al. 1998. Oncogene17, 1069. Kubbutat, M.H. and Vousden, K.H. 1997. Mol. Cell Biol.17, 460. Molinari, M. and Carafoli, E. 1997. J. Membr. Biol.156, 1. Sorimachi, H., et al. 1997. Biochem. J.328, 721. Squier, M.K. and Cohen, J.J. 1997. J. Immunol.158, 3690. Aoki, K., et al. 1986. FEBS Lett. 205, 313. Ohno, S., et al. 1986. Nucleic Acid Res.14, 5559.
Product Information
Detection method
Fluorometric
Form
96 Tests
Format
96-well plate
Kit contains
Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol.
Positive control
Calpain 1
Applications
Biological Information
Assay time
1.5 h
Sample Type
Cell lysates, plasma, serum
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Multiple Storage Conditions
Toxicity
Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
Multiple storage requirements
Storage Conditions
Upon arrival, store the Control Human Calpain 1 at -70°C and the remaining components at -20°C. With the exception of the Control Human Calpain 1, all components, once opened, can be stored at 4°C for up to 1 month.
Protect from Light
Protect from light
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol.
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