A sensitive fluorogenic assay kit for the determination of β-secretase (BACE) activity (Excitation max: 335-355 nm; Emission max: 495-501 nm). β-Secretase is a transmembrane aspartyl protease that cleaves membrane-bound amyloid precursor protein.
Catalogue Number
565785
Brand Family
Calbiochem®
Synonyms
BACE Activity Assay Kit, Fluorogenic
Application Data
The activity of recombinant was measured using the β-Secretase Substrate, &alpha-secretase substrate (not included), and γ-secretase substrate (not included) as outlined in the Detailed Protocol above.
References
Product Information
Detection method
Fluorogenic
Form
100 Tests
Format
Cuvette or 96-well plate
Kit contains
Extraction Buffer, Reaction Buffer, β-Secretase Substrate, β-Secretase Protein (Positive Control), β-Secretase Inhibitor, and a user protocol.
Applications
Biological Information
Assay time
1.5 h
Sample Type
Cell lysates, tissue extracts, purified BACE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended use
The Calbiochem® β-Secretase Activity Assay Kit, Fluorogenic, is intended for measuring the β-secretase (BACE) activity in cell lysates, tissue extracts, or purified enzyme preparations.
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
-20°C
Storage Conditions
Upon arrival store the entire contents of the kit at -20°C. Following reconstitution of the Active β-Secretase, aliquot and freeze (-70°C) to avoid loss of activity. Following initial thaw of the kit contents, store the Extraction Buffer and 2X Reaction Buffer at 4°C.
Protect from Light
Protect from light
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Extraction Buffer, Reaction Buffer, β-Secretase Substrate, β-Secretase Protein (Positive Control), β-Secretase Inhibitor, and a user protocol.
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