ImmunoPenTM

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Qiagen 凯杰 Biosharp Omega

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首页细胞生物学检测Millipore 密理博>

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市场价：: 0.00

促销价：: ¥0.00

货号：402176-1EA

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

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工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

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商品介绍
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Replacement Information

重要规格表

Description
Overview	Designed to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
Catalogue Number	402176
Brand Family	Calbiochem®

References

Product Information
Declaration	Not available for sale in Japan.

Applications
Application Notes	Frozen Sections Immunostaining Barrier Paraffin Sections
Application Comments	To start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip. Paraffin Section Method: Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed. Frozen Section Method: Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.

Applications

Application Notes

Frozen Sections
Immunostaining Barrier
Paraffin Sections

Application Comments

To start the ImmunoPen™ shake well and gently press the tip straight down several times until the fluid starts soaking the tip.

Paraffin Section Method:
Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen™. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen™ barrier after the digestion is completed.
Frozen Section Method:
Apply the ImmunoPen™ circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+15°C to +30°C
Do not freeze	Ok to freeze
Special Instructions	Keep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.