Non-Interfering Protein Assay™ Kit

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Non-Interfering Protein Assay™ Kit

市场价：: 0.00

促销价：: ¥0.00

货号：488250-1KTT

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

Description
Overview	An easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed. Protein concentration is based on the specific binding of copper to the peptide backbone. As the protein concentration increases, the concentration of unbound copper ions decreases, and the color density is inversely related to the amount of protein present in solution.
Catalogue Number	488250
Brand Family	Calbiochem®
Application Data
Materials Required but Not Delivered	• 2 ml tubes

References
References	Ho, T.H. et al. 2005. Human Molecular Genetics 14, 1539. Ladd, A.N. et al. 2001. Mol. Cell. Biol. 21, 1285. Loeb, D.M. et al. 2002. J. Biol. Chem. 277, 19627. Shiels, A. et al. 2007. Invest. Ophthalmol. Vis. Sci. 48, 500. Gushwa, N.N. et al. 2003. Plant Physiology 132, 1925. DePinto, W. et al. 2006. Mol. Cancer Ther. 5, 2644 Werner, M.E. et al. 2007. J. Biol. Chem. 282, 5560. Dennison, S.M. et al (2006). Biophysical Journal 90, 1661. Reeve, I. et al. 2002. PNAS 99, 8608.

References

Ho, T.H. et al. 2005. Human Molecular Genetics 14, 1539.
Ladd, A.N. et al. 2001. Mol. Cell. Biol. 21, 1285.
Loeb, D.M. et al. 2002. J. Biol. Chem. 277, 19627.
Shiels, A. et al. 2007. Invest. Ophthalmol. Vis. Sci. 48, 500.
Gushwa, N.N. et al. 2003. Plant Physiology 132, 1925.
DePinto, W. et al. 2006. Mol. Cancer Ther. 5, 2644
Werner, M.E. et al. 2007. J. Biol. Chem. 282, 5560.
Dennison, S.M. et al (2006). Biophysical Journal 90, 1661.
Reeve, I. et al. 2002. PNAS 99, 8608.

Product Information
Detection method	Colorimetric
Form	500 Tests
Format	Cuvette or 2 ml, 96-deep-well plate
Kit contains	UPPA™ Reagents I and II, Copper Solution I, Color Agents A and B, BSA Standard, and a user protocol.

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

Product Usage Statements
Intended use	The Non-Interfering Protein Assay™ is a highly sensitive colorimetric assay that overcomes interference by common laboratory agents. The assay removes detergents (non-ionic, ionic and zwitterionic), reducing agents (β-mercaptoethanol, DTT), chelating agents (EDTA), amines (Tris), sugars, and is highly tolerant of strong chaotropic buffers. It is suitable for determining protein concentrations in protein loading buffer (Laemmeli buffer), high β-mercaptoethanol concentrations (<15%), and in lipid and vesicle preparations. The Non-Interfering Protein Assay™ is linear between 0.5-50 µg and requires a small sample (1-50 µl).

Product Usage Statements

Intended use

The Non-Interfering Protein Assay™ is a highly sensitive colorimetric assay that overcomes interference by common laboratory agents. The assay removes detergents (non-ionic, ionic and zwitterionic), reducing agents (β-mercaptoethanol, DTT), chelating agents (EDTA), amines (Tris), sugars, and is highly tolerant of strong chaotropic buffers. It is suitable for determining protein concentrations in protein loading buffer (Laemmeli buffer), high β-mercaptoethanol concentrations (<15%), and in lipid and vesicle preparations. The Non-Interfering Protein Assay™ is linear between 0.5-50 µg and requires a small sample (1-50 µl).

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	+15°C to +30°C
Storage Conditions	Upon arrival, store UPPA-I and UPPA-II at room temperature and the remaining components at 4°C, in the dark in the original box.
Do not freeze	Ok to freeze