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Qiagen 凯杰 Biosharp Omega

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Nitric Oxide Synthase Assay Kit, Colorimetric

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号:482702-1KIT

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Detection Methods
Colorimetric
Description
Overview Assay kit useful for the rapid and accurate measurement of nitric oxide synthase (NOS) activity. This colorimetric assay uses lactate dehydrogenase (LDH) to destroy excess NADPH (cofactor for NOS) which interferes with the chemistry of Griess Reagent, commonly used for nitrite/nitrate detection. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.
Catalogue Number 482702
Brand Family Calbiochem®
Synonyms NOS Assay Kit, Colorimetric
Materials Required but Not Delivered A plate reader with 540 nm filter
An adjustable pipettor
Glass distilled water or HPLC-grade water
References
References Nims, R.W., et al. 1995. Methods Enzymol. 7, 48.
Moncada, S. 1992. Acta Physiol. Scand. 145, 201.
Nathan, C. 1992. FASEB J. 6, 3051.
Green, L.C., et al. 1982. Anal. Biochem. 126, 131.
Product Information
Detection method Colorimetric
Form 96 Tests
Format 96-well plate
Kit contains Assay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol.
Applications
Biological Information
Assay time 2 h
Sample Type Plasma, serum, urine, tissue culture medium, and tissue homogenates
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Storage Conditions Upon arrival store the entire contents of the kit at -20°C.
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains Assay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol.
Specifications

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