Assay kit useful for the rapid quantitative measurement of nitric oxide synthase (NOS) activity. It is based on the conversion of radioactive L-arginine to L-citrulline by NOS. Radioactive citrulline is separated from unreacted arginine on a cation-exchange resin and quantitated. Note: It is necessary to check the radiopurity of 3H-arginine before proceeding to use the kit.
Catalogue Number
482700
Brand Family
Calbiochem®
Synonyms
NOS Assay Kit
Materials Required but Not Delivered
•3H-Arginine monohydrochloride (40-70 Ci/mmol, 1 µCi/µl, Amersham, Arlington Heights, Illinois, Cat. No. TRK698) or 14C-Arginine monohydrochloride (~300 mCi/mmol, 50 µCi/ml (Amersham, Arlington Heights, Illinois, Cat. No. CFB63) • 10 mM Tris-HCl, pH 7.4 • Scintillation fluid and vials
References
References
Bredt, D.S., and Snyder, S.H. 1994. Annu. Rev. Biochem.63, 175. Kobzik, L., et al. 1994. Nature372, 546. Moncada, S., and Higgs, A. 1994. N. Engl. J. Med.329, 2002. Nathan, C., and Xie, Q.W. 1994. J. Biol. Chem.269, 13725. Busconi, L., and Michel, T. 1993. J. Biol. Chem.268, 8410. Marletta, M.A. 1993. J. Biol. Chem.268, 12231. Schmidt, H.H., et al. 1993. Biochim. Biophys. Acta1178, 153. Ignarro, L.J. 1991. Biochem. Pharmacol.41, 485. Pollock, J.S., et al. 1991. Proc. Natl. Acad. Sci. U.S.A88, 10480. Schmidt, H.H., et al. 1991. Proc. Natl. Acad. Sci. U.S.A88, 365. Slater, M., et al. 1991. FEBS Lett.291, 145. Stuehr, D.J., et al. 1991. Proc. Natl. Acad. Sci. U.S.A88, 7773. Bredt, D.S., and Snyder, S.H. 1990. Proc. Natl. Acad. Sci. USA87, 689. Bredt, D.S., and Snyder, S.H. 1989. Proc. Natl. Acad. Sci. USA86, 9030. Knowles, R.G., et al. 1989. Proc. Natl. Acad. Sci. U.S.A86, 5159.
Product Information
Detection method
Radiometric
Form
50 Tests
Format
Scintillation vial
Kit contains
Homogenization Buffer, Reaction Buffer, NADPH, CaCl₂, Calmodulin, Stop Solution, Rat Brain Cerebellum Extract, NG-Nitro-L-Arginine Methyl Ester (L-NAME), Equilibrated Resin, Spin Cups, Cup Holders, and a user protocol. Radioactive arginine is not provided with this kit.
Applications
Biological Information
Assay range
2 h
Sample Type
Tissue or cell extracts
Physicochemical Information
Sensitivity
picomolar range
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 22-36/37/38
Harmful if swallowed. Irritating to eyes, respiratory system and skin.
S Phrase
S: 26-36
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Product Usage Statements
Intended use
The Calbiochem® Nitric Oxide Synthase (NOS) Assay Kit is a simple, sensitive and specific assay for nitric oxide synthase (NOS) activity. The NOS assay kit is based on the biochemical conversion of L-arginine to L-citrulline by NOS. Advantages of the NOS assay kit include the use of radioactive substrates (³H-arginine or ¹⁴C-arginine), and an easy method for separating neutrally charged citrulline from positively charged arginine. This kit has sensitivity to the picomole level and allows the assay of up to fifty samples in less than 2 h.
Storage and Shipping Information
Ship Code
Multiple Storage Conditions
Toxicity
Multiple Toxicity Values, refer to MSDS
Storage
Multiple storage requirements
Storage Conditions
Upon arrival store the Homogenization Buffer, Stop Buffer, Equilibrated Resin, CaCl₂, Elution Buffer, and Spin Cups and Cup Holders at room temperature; store the Rat Brain Cerebellum Extract, Calmodulin, Reaction Buffer, NADPH, and L-NAME, HCl at -70°C.
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Homogenization Buffer, Reaction Buffer, NADPH, CaCl₂, Calmodulin, Stop Solution, Rat Brain Cerebellum Extract, NG-Nitro-L-Arginine Methyl Ester (L-NAME), Equilibrated Resin, Spin Cups, Cup Holders, and a user protocol. Radioactive arginine is not provided with this kit.
新浪微博
腾讯微博
QQ空间
客服
