Replacement Information |
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Detection Methods |
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Fluorometric |
Description | |
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Overview | Assay kit useful for the rapid quantitative measurement of nitric oxide (NO). Displays 50-fold increased sensitivity over the colorimetric nitric oxide assay kit (Cat. No. 482650). The assay is based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the addition of 2,3-diaminonapthalene (DAN), and NaOH, which converts nitrite to a fluorescent compound. Fluorescence measurements of this compound accurately determine the nitrite (NO2-) concentration (excitation max.: 365 nm; emission max.: 450 nm). Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI. |
Catalogue Number | 482655 |
Brand Family | Calbiochem® |
Materials Required but Not Delivered | • A fluorometric plate reader capable of measuring fluorescence (excitation = 365 nm; emission = 450 nm) • An adjustable pipette • Glass distilled or HPLC-Grade water |
References | |
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References | Miles, A.M., et al. 1996. Methods Enzymol. 268, 105. Misko, T.P., et al. 1993. Anal. Biochem. 214, 11. Green, L.C., et al. 1982. Anal. Biochem. 126, 131. |
Applications |
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Biological Information | |
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Assay time | 3-5 h |
Sample Type | Aqueous solutions |
Physicochemical Information |
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Dimensions |
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Materials Information |
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Toxicological Information |
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Safety Information according to GHS |
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Product Usage Statements |
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Packaging Information |
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Transport Information |
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Supplemental Information | |
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Kit contains | Assay Buffer, Nitrate Reductase, Enzyme Cofactors, Nitrate Standard, Nitrite Standard, DAN Reagent, NaOH, Microtiter Plates, Plate Covers, and a user protocol. |
Specifications |
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