Nitric Oxide Assay Kit, Colorimetric - 炼石商城

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Nitric Oxide Assay Kit, Colorimetric

市场价：: 0.00

促销价：: ¥0.00

货号：482650-1KTT

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Detection Methods
Colorimetric

Description
Overview	Assay kit for the rapid quantitative measurement of total nitric oxide (NO). Based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the spectrophotometric quantitation of nitrite levels using Griess Reagent. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.
Catalogue Number	482650
Brand Family	Calbiochem®
Materials Required but Not Delivered	• Distilled or deionized water • Microplate reader with 540 nm filter. NOTE: The wavelength of the filter can be 530 to 560 nm, however, maximum absorbance occurs at 540 nm. • Precision pipettes ranging from 1 µl to 100 µl, and disposable pipette tips. NOTE: If all 96 wells are to be used at one time, it is suggested that a multichannel pipettor be used. • Clean test tubes • Vortex mixer

References
References	Nims, R.W., et al. 1996. Methods Enzymol. 268, 93.

Product Information
Detection method	Colorimetric
Form	80 Tests
Format	96-well plate
Kit contains	Recombinant Zea mays Nitrate Reductase, NADH, Nitrate Standard, Color Reagents, Buffer, 96-Well Plate, and a user protocol.

Applications

Biological Information
Assay time	1-1.5 h
Sample Type	Aqueous solutions

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 34-36/37/38-40-42/43-20/21/22 Causes burns. Irritating to eyes, respiratory system and skin. Limited evidence of a carcinogenic effect. May cause sensitization by inhalation and skin contact. Harmful by inhalation, in contact with skin and if swallowed.
S Phrase	S: 26-36-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

Product Usage Statements
Intended use	This Calbiochem^® Nitric Oxide Assay Kit, Colorimetric is intended for the spectrophotometric measurement of the nitric oxide (NO) produced in in vitro experimental systems. This method can be used to accurately measure as little as 1 µM (pmol/ml) NO in aqueous solutions and requires small quantities of samples (5 to 85 µl, depending on the concentration of nitric oxide in the sample).

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	+2°C to +8°C
Storage Conditions	Upon arrival immediately store the Nitrate Reductase at -20°C immediately and all other components at 4°C.
Protect from Light	Protect from light
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information
Kit contains	Recombinant Zea mays Nitrate Reductase, NADH, Nitrate Standard, Color Reagents, Buffer, 96-Well Plate, and a user protocol.

Specifications

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