A set of four different monoclonal antibodies specific for serine phosphorylation sites. Recognition is dependent on phosphorylation and the surrounding amino acid motif. Recognizes serine phosphorylated proteins in Arabidopsis, bacteria, chicken, human, maize, mouse, rat, tobacco, Xenopus, yeast, and zebrafish. Phosphorylation patterns in cell extracts may differ when probed with different antibodies.
Catalogue Number
525282
Brand Family
Calbiochem®
Application Data
Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Whole cell lysate from A431 cells treated with pervanadate (all lates). Primary antibodies: PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (1C8) (Cat. No. 525281) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A3) (Cat. No. 525283) (1 µg/ml), and PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A9) (Cat. No. 525284) (1 µg/ml). Detection: chemiluminescence. Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibodies: PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (1C8) (Cat. No. 525281) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A3) (Cat. No. 525283) (1 µg/ml), and PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A9) (Cat. No. 525284) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody.
References
Product Information
Form
Lyophilized
Formulation
25 µg each antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl control phosphoproteins lyophilized 20 mM Na₂HPO₄, 0.1% SDS.
Kit contains
25 μg of each of the Phosphoserine Antibodies including clones 1C8, 4A3, 4A9, and 16B4, Rabbit Muscle Positive Control, and a data sheet.
Positive control
Phosphoproteins purified from rabbit muscle (included)
Immunoblotting (see characteristics table) Immunoprecipitation (see characteristics table) ELISA (see characteristics table)
Application Comments
DO NOT use milk or casein for membrane blocking or antibody dilutions; use BSA. For immunoblotting with the positive control, use 20 µl/lane (minigel) for colorimetric detection of 5 µl/lane for chemiluminescent detection. The phosphorylation patterns in a given cell lysate may differ when probed with individual antibodies due to sequence specificity. Antibodies should be titrated for optimal results in individual systems.
Biological Information
Immunogen
phosphoserine-containing peptides or phosphoserine conjugated to KLH
Host
Mouse
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute each antibody with 1 ml H₂O (15 min, room temperature). Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C. Thaw aliquots at 37°C prior to use. Reconstitute the positive control with 200 µl H₂O. After complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate for 5 min at 90°C. Following incubation, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of all solutions.
Packaging Information
Transport Information
Supplemental Information
Kit contains
25 μg of each of the Phosphoserine Antibodies including clones 1C8, 4A3, 4A9, and 16B4, Rabbit Muscle Positive Control, and a data sheet.
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