OxyDNA Assay Kit, Fluorometric

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OxyDNA Assay Kit, Fluorometric

市场价：: 0.00

促销价：: ¥0.00

货号：500095-1KIT

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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工作时间

周一至周五：9:00-18:00

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0771-3293894

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Replacement Information

Description
Overview	This product has been discontinued. Assay kit used to detect oxidative damage to DNA in vitro. Based on the direct binding of a fluorescent probe to the DNA adduct 8-oxoguanine, a major oxidation product and an important indicator of free radical-induced DNA damage and oxidative stress. The FITC-conjugate binds to 8-oxoguanine in damaged cells and fluorescence is monitored using flow cytometery (excitation 495 nm, emission 515 nm).
Catalogue Number	500095
Brand Family	Calbiochem®
Materials Required but Not Delivered	• Micropipettes: 20 µl to 100 µl, 200 µl to 1000 µl • Test tubes • 1 L beaker • Graduated cylinder • Reagents for fixing and permeabilising cells • Deionized/Distilled water • 37°C water bath • Flow cytometer with appropriate filter combination for FITC (excitation filter 495 nm, barrier filter 515 nm)

References
References	Silva, P.F.N. et al. 2007. Theriogenology 67, 609. Babbar, N. and Casero, Jr., R.A. 2006. Cancer Res. 66, 11125. Lai, K.N., et al. 2006. Nephrol. Dial. Transplant. 21, 1188. Sampson, M.J. et al. 2006. Diabetes Care 29, 283. Maniscalco, W.M., et al. 2005. Pediatric Res. 58, 594. Osella, D., et al. 2005. Inorganica Chimica Acta 358, 1993. Adamek B et al. 2004. Polish Journal of Environmental Studies 13 Suppl. II, 29. Roper, J.M., et al. 2004. Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1045. Ogawa, Y. et al. 2003. International Journal of Molecular Medicine 11, 27. Ogawa, Y. et al. 2003. International Journal of Molecular Medicine 11, 149. Sparrow J.R. et al. 2003. Investigative Ophthalmology and Visual Science (14)5, 2245. Tao Peng, H-M. et al. 2003. World J Gastroenterol 9, 2186. Riemschneider, S., et al. 2002. Acta Derm. Venereol. 82, 325. de Zwart, L.L., et al. 1999. Free Radic. Biol. Med. 26, 202. Tsurudome, Y., et al. 1999. Carcinogenesis 20, 1573. Inoue, M., et al. 1998. Jpn. J. Cancer Res. 89, 691. Kasai, H. 1997. Mutat. Res. 387, 147. Cooke, C., et al. 1996. Toxicol. Ecotoxicol. News 3, 101. Selected Citations Roper, J. M., et al. 2004. Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1045. Babbar, N., and Casero, R. A., Jr., 2006. Cancer Res. 66, 23.

References

Silva, P.F.N. et al. 2007. Theriogenology 67, 609.
Babbar, N. and Casero, Jr., R.A. 2006. Cancer Res. 66, 11125.
Lai, K.N., et al. 2006. Nephrol. Dial. Transplant. 21, 1188.
Sampson, M.J. et al. 2006. Diabetes Care 29, 283.
Maniscalco, W.M., et al. 2005. Pediatric Res. 58, 594.
Osella, D., et al. 2005. Inorganica Chimica Acta 358, 1993.
Adamek B et al. 2004. Polish Journal of Environmental Studies 13 Suppl. II, 29.
Roper, J.M., et al. 2004. Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1045.
Ogawa, Y. et al. 2003. International Journal of Molecular Medicine 11, 27.
Ogawa, Y. et al. 2003. International Journal of Molecular Medicine 11, 149.
Sparrow J.R. et al. 2003. Investigative Ophthalmology and Visual Science (14)5, 2245.
Tao Peng, H-M. et al. 2003. World J Gastroenterol 9, 2186.
Riemschneider, S., et al. 2002. Acta Derm. Venereol. 82, 325.
de Zwart, L.L., et al. 1999. Free Radic. Biol. Med. 26, 202.
Tsurudome, Y., et al. 1999. Carcinogenesis 20, 1573.
Inoue, M., et al. 1998. Jpn. J. Cancer Res. 89, 691.
Kasai, H. 1997. Mutat. Res. 387, 147.
Cooke, C., et al. 1996. Toxicol. Ecotoxicol. News 3, 101.

Selected Citations
Roper, J. M., et al. 2004. Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1045.
Babbar, N., and Casero, R. A., Jr., 2006. Cancer Res. 66, 23.

Product Information
Detection method	Flow cytometry or fluorescence microscopy
Form	50 Tests
Kit contains	Wash Concentrate, Binding Protein-FITC Conjugate, and a user protocol.

Applications
Application References	Fluorescent Microscopy Lai, K.N., et al. 2006. Nephrol. Dial. Transplant. 21, 1188. Maniscalco, W.M., et al. 2005. Pediatric Res. 58, 594. Roper, J.M., et al. 2004. Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1045. Other Applications Babbar, N. and Casero, Jr., R.A. 2006. Cancer Res. 66, 11125. Osella, D., et al. 2005. Inorganica Chimica Acta 358, 1993. Riemschneider, S., et al. 2002. Acta Derm. Venereol. 82, 325.

Applications

Application References

Fluorescent Microscopy Lai, K.N., et al. 2006. Nephrol. Dial. Transplant. 21, 1188. Maniscalco, W.M., et al. 2005. Pediatric Res. 58, 594. Roper, J.M., et al. 2004. Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1045. Other Applications Babbar, N. and Casero, Jr., R.A. 2006. Cancer Res. 66, 11125. Osella, D., et al. 2005. Inorganica Chimica Acta 358, 1993. Riemschneider, S., et al. 2002. Acta Derm. Venereol. 82, 325.

Biological Information
Assay time	4 h
Sample Type	Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Intended use	The Calbiochem^® OxyDNA Kit is an in vitro fluorescent protein binding method for the detection of oxidative damage to DNA in fixed permeabilized cells using flow cytometry or fluorescence microscopy. Material of both human and animal origin can be tested.

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	+2°C to +8°C
Storage Conditions	Upon arrival store the entire contents of the kit at 4°C. Note: FITC conjugate must be stored in the dark.
Do not freeze	Ok to freeze