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Qiagen 凯杰 Biosharp Omega

首页细胞生物学检测Millipore 密理博>

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Superoxide Dismutase Assay Kit II

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价:
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号:574601-1KIT

牌:Millipore 密理博

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Replacement Information
Description
Overview A sensitive spectrophotometric assay (450 nm) for the measurement of all three types (Cu/Zn, Mn, and Fe) of SOD.
Catalogue Number 574601
Brand Family Calbiochem®
Materials Required but Not Delivered Plate reader capable of reading absorbance at 450 nm.
Adjustable pipettors and a repeat pipettor.
A source of pure water. Glass distilled water or HPLC-grade water is acceptable.
References
References Maier, C.M. and Chan, P.H 2002. The Neuroscientist 8, 323.
Mattiazz, M., et al. 2002. J. Biol. Chem. 277, 29626.
Beckman, J.S. and Koppenol, W.H. 1996. Am. J. Physiol. 271, C1424.
Liu, D. 1996. J. Mol. Neuro. 7, 159.
MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853.
Sun, E., et al. 1995. Biol. Trace Elem. Res. 48, 231.
Sandstrom, J., et al. 1994. J. Biol. Chem. 269, 19163.
Marklund, S. 1980. Acta Physiol. Scand. Suppl. 492, 19.
Malstrom, B. 1975. in The Enzymes. Boyer, P., editor. XIIB, Academic Press, New York, 533.
Product Information
Unit of Definition The amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.
Detection method Colorimetric
Form 96 Tests
Format 96-well plate
Kit contains Assay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
Positive control Bovine erythrocyte SOD (Cu/Zn)
Applications
Biological Information
Assay range 0.025-0.25 units per ml SOD
Assay time 1.5 h
Sample Type Plasma, serum, erythrocyte lysates, and other lysates, tissue homogenates, and cell lysates
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Storage Conditions Upon arrival store entire contents of the kit at -20°C.
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains Assay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
Specifications

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