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| Replacement Information |
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| 产品目录编号 | 库存情况 | 包装 | 数量 / 包装 | 价格 | 数量 | |
|---|---|---|---|---|---|---|
| 69078-3CN |
| 塑胶安瓿;塑胶针药瓶 | 25 blot |
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| Description | |
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| Overview | The Perfect Protein™ HRP Western Blot Kit includes an HRP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits. A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract. B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein. |
| Catalogue Number | 69078 |
| Brand Family | Novagen® |
| Features and benefits | • Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection. • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection. • Can be used with colorimetric and chemiluminescent AP or HRP substrates. • Recombinant, unmodified markers give sharp, accurately sized bands. • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection. • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa. |
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| Form | Liquid |
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| Ship Code | Blue Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | -20°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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