A kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
Catalogue Number
539125
Brand Family
Calbiochem®
Materials Required but Not Delivered
• Trichloroacetic acid (TCA), 5% • Spectrophotometer that can accurately measure the absorbance at 492 nm • Pipetting devices for accurate delivery of volumes required for the assay • Cuvettes with optical properties suitable for use at 492 nm • A constant-temperature water bath set at 37°C • Microfuge for generating approximately 12,000 x g
References
References
Twining, S.S. 1984. Anal. Biochem.143, 30. Wiesner, R., et al. 1982. Anal. Biochem.121, 290.
Product Information
Detection method
Colorimetric
Form
200 Tests
Format
Cuvette or 96-well plate
Kit contains
A Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
Applications
Biological Information
Assay time
3 h
Sample Type
Biological samples
Physicochemical Information
Sensitivity
10 ng/ml
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Storage
-20°C
Storage Conditions
• FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light. • Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C. • Incubation buffer and assay buffer may be stored at 4°C or -20°C.
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
A Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
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