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Description | |
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Overview | A rapid method for isolating ubiquitinated proteins using affinity beads comprised of a GST-fusion protein containing an ubiquitin-associated sequence bound to glutathione-agarose. Useful for the enrichment of polyubiquitinated proteins from cell and tissue lysates of a broad range of species including canine, human, mouse, and yeast. The ubiquitinated proteins can be identified by loading the beads directly onto SDS-PAGE and then immunoblotting with the antibody of choice or Anti-Ubiquitin (Cat. No. 662099). Alternatively, it is possible that the beads can be treated with Isopeptidase T (Cat. No. 419700) to release the proteins from the ubiquitin chains. |
Catalogue Number | 662200 |
Brand Family | Calbiochem® |
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References | Chen, L. and Madura, K. 2002. Mol. Cell Biol. 22, 4902. Chen, L., et al. 2001. EMBO Rep. 2, 933. |
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Kit contains | Polyubiquitin Affinity Beads, Control Glutathione-Agarose Beads, Control Lysate, and a user protocol. |
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