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Qiagen 凯杰 Biosharp Omega

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Ubiquitinated Protein Enrichment Kit

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号:662200-1KIT

牌:Millipore 密理博

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Replacement Information
Description
Overview A rapid method for isolating ubiquitinated proteins using affinity beads comprised of a GST-fusion protein containing an ubiquitin-associated sequence bound to glutathione-agarose. Useful for the enrichment of polyubiquitinated proteins from cell and tissue lysates of a broad range of species including canine, human, mouse, and yeast. The ubiquitinated proteins can be identified by loading the beads directly onto SDS-PAGE and then immunoblotting with the antibody of choice or Anti-Ubiquitin (Cat. No. 662099). Alternatively, it is possible that the beads can be treated with Isopeptidase T (Cat. No. 419700) to release the proteins from the ubiquitin chains.
Catalogue Number 662200
Brand Family Calbiochem®
References
References Chen, L. and Madura, K. 2002. Mol. Cell Biol. 22, 4902.
Chen, L., et al. 2001. EMBO Rep. 2, 933.
Product Information
Declaration Sold under license of PCT Application wo 03/049,602.
Form 1 kit sufficient to process 12.5-25 mg lysate
Kit contains Polyubiquitin Affinity Beads, Control Glutathione-Agarose Beads, Control Lysate, and a user protocol.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase R: 23/24-28-32-42-50/53

Toxic by inhalation and in contact with skin.
Very toxic if swallowed.
Contact with acids liberates very toxic gas.
May cause sensitization by inhalation.
Very toxic to aquatic organisms; may cause long-term adverse effects in the aquatic environment.
S Phrase S: 26-27-28-36/37/39-45-60-61

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.

Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
This material and its container must be disposed of as hazardous waste.
Avoid release to the environment. Refer to special instructions/safety data sheet.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Storage Conditions Upon arrival store entire contents of the kit at 4°C.
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Kit contains Polyubiquitin Affinity Beads, Control Glutathione-Agarose Beads, Control Lysate, and a user protocol.
Specifications

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