GSH/GSSG Ratio Assay Kit

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GSH/GSSG Ratio Assay Kit

市场价：: 0.00

促销价：: ¥0.00

货号：371757-1KIT

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Detection Methods
Colorimetric

Description
Overview	A spectrophotometric assay kit used for the measurement of reduced to oxidized glutathione ratio (GSH/GSSG) in biological samples.
Catalogue Number	371757
Brand Family	Calbiochem®
Features and benefits	• Highly Sensitive and Specific: Lower limit of detection in sample mixture is 9 nM GSSG. Over 13 different compounds were tested to assess any interference and exhibited no interference. • Easy to Use: A simplified colorimetric assay. the GSH/GSSG assay uses the thiol-scavenging reagent that rapidly scavenges GSH, but does not interfere with glutathione reductase assay. Each kit can be used for up to 100 tests for determining total glutathione/GSSG ratios or 200 tests for either of the two. Assay time: About 1.5 h • Versatile: Can be used with whole blood, cell lysates, and tissue extracts.
Application Data	A four point calibration curve A₄₁₂/min VS. µ GSH used to determine the concentration of GSSG. Stability of GSSG, from a rested subject, in the intact red blood cell at 4°C. (GSH-159). Stability of GSSG in the red blood cell lysate at 4°C. (GSH-161)
Materials Required but Not Delivered	• Spectrophotometer • Centrifuge 1000 x g or greater • Spectrophotometric cuvettes with a 1 cm optical path length (glass or polystyrene). • Disposable tubes and stoppers (glass or polypropylene). • Pipettes, preferably adjustable capable of accurately pipetting 10, 50, 100, 200, 700 and 3000 µl. • Balance • Metaphosphoric acid

References
References	Anderson, M. 1996. Glutathione in Free Radicals, A Practical Approach, ed. N.A. Punchard and F.J. Kelly Oxford University Press, New York, p 213. Richie, J.P. Jr., et al. 1996. Clinical Chemistry 42, 64. Griffith, O.W. 1980. Analytical Biochemistry 106, 207. Tietze, F. 1969. Analytical Chemistry 27, 502. Guntherberg, H., and Rost, J. 1966. Analytical Biochemistry 15, 205.

References

Anderson, M. 1996. Glutathione in Free Radicals, A Practical Approach, ed. N.A. Punchard and F.J. Kelly Oxford University Press, New York, p 213.
Richie, J.P. Jr., et al. 1996. Clinical Chemistry 42, 64.
Griffith, O.W. 1980. Analytical Biochemistry 106, 207.
Tietze, F. 1969. Analytical Chemistry 27, 502.
Guntherberg, H., and Rost, J. 1966. Analytical Biochemistry 15, 205.

Product Information
Detection method	Colorimetric
Form	200 Tests
Format	Cuvette
Kit contains	Chromogen DTNB, Glutathione Reductase Enzyme, NADPH, Scavenger, Assay Buffer, GSSG Buffer, Standard, and a user protocol. Each kit can be used to perform 100 tests representing the ratio of total Glutathione to the oxidized form or 200 tests of either.

Applications

Biological Information
Assay time	1.5 h
Sample Type	cell lysates, tissue extracts, blood

Physicochemical Information
Sensitivity	9 nM GSSG

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Storage Conditions	When not in use, place the bottles at 4°C. Do not allow the capped reagent bottles to sit at room temperature for long periods of time.
Protect from Light	Protect from light
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information
Kit contains	Chromogen DTNB, Glutathione Reductase Enzyme, NADPH, Scavenger, Assay Buffer, GSSG Buffer, Standard, and a user protocol. Each kit can be used to perform 100 tests representing the ratio of total Glutathione to the oxidized form or 200 tests of either.