Native cathepsin D from human liver. A major lysosomal aspartyl protease produced as a 52 kDa proenzyme. Overexpression of cathepsin D in human breast cancers is associated with higher risk of relapse and metastasis. Cathepsin D degrades extracellular matrix and releases growth factors bound to the matrix.
Catalogue Number
219401
Brand Family
Calbiochem®
References
References
Berchem, G., et al. 2002. Oncogene21, 5951. Barbi, G.P., et al. 1994. Oncology51, 329. Krishnan, V., et al. 1994. J. Biol. Chem.269, 15912. Touitou, I., et al. 1994. Eur. J. Cancer30A, 390. Rijnboutt, S., et al. 1991. J. Biol. Chem.266, 23586. Johnson, G.V., et al. 1991. J. Neurochem.57, 1577. Erickson, A. 1989. J. Cell. Biochem.40, 31.
Product Information
CAS number
9025-26-7
Unit of Definition
One unit is defined as the amount of enzyme that will digest acid-denatured hemoglobin in 60 min, at 37°C, pH 3.3, releasing peptides soluble in 10% TCA, as measured by an increase in absorbance of 1.0 at 280 nm
EC number
3.4.23.5
Form
Lyophilized
Formulation
Lyophilized from 2 mM sodium phosphate buffer, pH 6.5.
Applications
Biological Information
Purity
≥90% by SDS-PAGE
Source
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity
≥300 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C. Keep on ice when working with material. When product is brought to its active pH (3.3), autolysis occurs.