PKA, Catalytic Subunit, Bovine Heart - 炼石商城

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PKA, Catalytic Subunit, Bovine Heart

市场价：: 0.00

促销价：: ¥0.00

货号：539576-25UG

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

Description
Overview	Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro* with purified substrates or following its introduction into intact cells or cell extracts.
Overview	Note: 25 µg ≥ 500,000 units.
Catalogue Number	539576
Brand Family	Calbiochem®
Synonyms	cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
Application Data

References
References	Corbin, J.D., et al. 1988. Methods Enzymol 159, 74. Rannels, S.R., et al 1983. Methods Enzymol 99, 55. Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.

Product Information
Unit of Definition	One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
Form	Liquid
Formulation	In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

Applications

Biological Information
Purity	≥90% by SDS-PAGE
Specific Activity	≥20,000 units/µg protein
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C).

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