Replacement Information |
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产品目录编号 | 库存情况 | 包装 | 数量 / 包装 | 价格 | 数量 | |
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70839-3CN |
| 塑胶安瓿;塑胶针药瓶 | 0.4 ml |
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70839-4CN |
| 玻璃瓶 | 1 ml |
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Description | |
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Overview | Origami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. |
Genotype: F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB pLysS (CamR, KanR, TetR) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
Catalogue Number | 70839 |
Brand Family | Novagen® |
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Product Information | |
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Components | |
Guaranteed efficiency | Guaranteed efficiency: > 2 x 10⁶ cfu/μg |
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Safety Information |
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Product Usage Statements |
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Storage and Shipping Information | |
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Ship Code | Dry Ice Only |
Toxicity | Multiple Toxicity Values, refer to MSDS |
Storage | ≤ -70°C |
Do not freeze | Ok to freeze |
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Supplemental Information |
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Specifications |
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