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| Replacement Information |
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| 产品目录编号 | 库存情况 | 包装 | 数量 / 包装 | 价格 | 数量 | |
|---|---|---|---|---|---|---|
| 70777-3CN |
| 玻璃瓶 | 1 ea |
| — |
| Description | |
|---|---|
| Overview | pET Expression Systems and pET pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression. Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include: • 10 µg pET vector DNA (for each indicated plasmid) • 0.2 ml BL21 Glycerol Stock • 0.2 ml BL21(DE3) Glycerol Stock • 0.2 ml BL21(DE3)pLysS Glycerol Stock • 0.2 ml Induction Control Glycerol Stock Components: pET Expression Systems plus Competent Cells pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description: • 0.2 ml NovaBlue Competent Cells • 0.2 ml BL21(DE3) Competent Cells • 0.2 ml BL21(DE3)pLysS Competent Cells • 2 × 0.2 ml SOC Medium • 10 µl Test Plasmid These components are sufficient for up to 10 transformations in each host. Purification and Detection Reagents Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters. pET Expression System 28 The pET Expression System 28 contains 10 µg each of the four versions of pET-28 (pET-28a–c(+)). The pET-28a–c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB074VM. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product. |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 70777 |
| Brand Family | Novagen® |
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| Ship Code | Shipped with Blue Ice or with Dry Ice |
| Toxicity | Standard Handling |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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