Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
Catalogue Number
324682
Brand Family
Calbiochem®
References
References
Largman, C. et al. 1976. Biochemestry15, 2491. Mandl, I. 1962. Methods in Enzymol.5, 665.
Product Information
CAS number
39445-21-1
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
EC number
3.4.21.36
Form
Lyophilized
Formulation
Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
Applications
Biological Information
Specific Activity
≥25 units/mg protein
Physicochemical Information
Contaminants
Chymotrypsin: ≤0.2%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
TS5052000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Protect from Moisture
Protect from moisture
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
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