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Qiagen 凯杰 Biosharp Omega

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Human Ig-Primer Set

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号:69830-3

牌:Millipore 密理博

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      Description
      Overview The Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.

      In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.

      Please call Technical Service if you need more information regarding individual Ig-Primer Sets.

      Catalogue Number 69830
      Brand Family Novagen®
      References
      References Larrick, J.W., et al. 1989. Biochem. Biophys. Res.
      Comm.
      160, 1250. Jones, S.T. and Bendig, M.M. 1991.
      Biotechnology 9, 88.
      Product Information
      500 pmol Hu IgM VH 3ʹ-1 Primer
      500 pmol Hu IgG VH 3ʹ-1 Primer
      500 pmol Hu Ig VH 5ʹ-A Primer
      500 pmol Hu Ig VH 5ʹ-B Primer
      500 pmol Hu Ig VH 5ʹ-C Primer
      500 pmol Hu Igκ VL 3ʹ-1 Primer
      500 pmol Hu Igκ VL 5ʹ-A Primer
      500 pmol Hu Igλ VL 3ʹ-1 Primer
      500 pmol Hu Igλ VL 5ʹ-A Primer
      10 µl Human Positive Control PCR Template
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

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