NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.
Source
Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration
5.0 U/µl
Endonuclease
None detected
Exonuclease
None detected
Amplification effiency
Functional PCR
Storage
–20°C
Catalogue Number71091 Brand Family Novagen® Features and benefits
Higher PCR specificity and yield
Improved low-copy target amplification
Ambient temperature setup compatible with automation
Target amplification of up to 5 kbp
Ideal for quantitative and high-throughput PCR applications
References
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.
•
250 U or 5 × 250 U
NovaTaq Hot Start DNA Polymerase (5 U/µl)
•
1.5 ml or 5 × 1.5 ml
10X NovaTaq Hot Start Buffer
•
1.5 ml or 5 × 1.5 ml
25 mM MgCl₂
Declaration
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Applications
Application
NovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase
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