A non-radioactive competitive immunoassay kit for the rapid quantitative measurement of cAMP. No neutralization, extraction, drying, or reconstitution of samples is required. Samples are treated with 0.1N HCl to lyse the cells, stop endogenous phosphodiesterase activity, and stabilize the released cAMP, which can then be analyzed directly in the microtiter plate.
Catalogue Number
116811
Brand Family
Calbiochem®
Materials Required but Not Delivered
• Deionized or distilled water. • Precision pipets for volumes between 5 µl and 1000 µl. • Repeater pipets for dispensing 50 µl and 200 µl. • Disposable beakers for diluting buffer concentrates. • Graduated cylinders. • A microplate shaker. • Adsorbent paper for blotting. • Microplate reader capable of reading at 405 nm, preferably with correction between 570 and 590 nm.
References
References
Chard, T. In: An Introduction to Radioimmunoassay and Related Techniques. 1990. 4th Ed., Elsevier, Amsterdam. NCCLS Evaluation Protocols, SC1, 1989, NCCLS, Villanova, PA, 19085. Tijssen, P. In: Practice and Theory of Enzyme Immunoassays. 1985. Elsevier, Amsterdam. Chabardes, D., et al. 1980. J. Clin. Invest.65, 439. Grill, V., and Cerasi, E. 1974. J. Biol. Chem.249, 41961. Szentivanyi, A. 1968. J. Allergy42, 203. Haynes, R.C. 1958. J. Biol. Chem.233, 1220.
Product Information
Detection method
Colorimetric
Form
100 Tests
Format
96-well format
Kit contains
One Antibody Coated 96-Well Plate, cAMP Direct Conjugate, Anti-cAMP, cAMP Standard, 0.1 N HCl, Neutralizing Reagent, Wash Buffer, Color Substrate, Stop Solution, Triethylamine, Acetic Anhydride, a Plate Sealer, and a user protocol.
Applications
Application References
Gettys, T.W., et al. 1991. J. Biol. Chem.266, 15949. Gettys, T.W., et al. 1990. 2nd Messengers & Phosphoprot.13, 37. Yamamoto, I., and Tsuji, J. 1981. Immunopharm. 3, 53. Collins, W.P., and Hennam, J.F. 1976. In: Molecular Aspects of Medicine Vol. 1, (Baum, H. and Cergeley, J., eds.) p. 3, Pergamon, England. Fausto, H., and Butcher, F.R. 1976. Biochim. Biophys. Acta428, 702. Farmer, R.W., et al. 1975. Anal. Biochem.64, 455. Steiner, A.L. 1974. Methods Enzymol.38, 96.
Highly flammable. Harmful by inhalation and in contact with skin. Very toxic if swallowed. Contact with acids liberates very toxic gas. Causes severe burns. Very toxic to aquatic organisms. May cause long-term adverse effects in the aquatic environment.
S Phrase
S: 26-3-16-27-28-29-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Keep in a cool place. Keep away from sources of ignition - No Smoking. Take off immediately all contaminated clothing.
Do not empty into drains. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended use
The Calbiochem® cAMP Direct Immunoassay Kit, Colorimetric is a competitive immunoassay for the quantitative assay of cAMP in cell lysates, tissue extracts, and serum/plasma.
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+2°C to +8°C
Storage Conditions
Upon arrival store the cAMP Direct Conjugate and the Cyclic AMP Standard at -20°C and the remaining components of the kit at 4°C.
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
One Antibody Coated 96-Well Plate, cAMP Direct Conjugate, Anti-cAMP, cAMP Standard, 0.1 N HCl, Neutralizing Reagent, Wash Buffer, Color Substrate, Stop Solution, Triethylamine, Acetic Anhydride, a Plate Sealer, and a user protocol.
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