Replacement Information |
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产品目录编号 | 库存情况 | 包装 | 数量 / 包装 | 价格 | 数量 | |
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71034-3CN |
| 塑胶安瓿;塑胶针药瓶 | 0.4 ml |
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71034-4CN |
| 玻璃瓶 | 1 ml |
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Description | |
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Overview | RosettaBlue™ host strains are NovaBlue derivatives that combine high transformation efficiency and recA endA lacIq mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In RosettaBlue(DE3)pLysS, the rare tRNA genes are present on the same plasmid that carries the T7 lysozyme gene. Blue/white screening is not possible with RosettaBlue(DE3) strains due to the presence of the lacZ α-peptide coding sequence in the λDE3 prophage. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. |
Genotype: endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac (DE3) F′[proA+B+ lacIqZΔM15::Tn10] LysSRARE (CamR, TetR) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
Catalogue Number | 71034 |
Brand Family | Novagen® |
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Product Information | |
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Components | |
Guaranteed efficiency | Guaranteed efficiency: > 1 x 10⁶ cfu/µg |
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Product Usage Statements |
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Storage and Shipping Information | |
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Ship Code | Dry Ice Only |
Toxicity | Standard Handling |
Storage | ≤ -70°C |
Do not freeze | Ok to freeze |
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