The unique TriEx™ Sf9 Cells are derived from a high-yielding clone of Sf9 cells. Pre-adapted for growth in Novagen serum-free TriEx Insect Cell Medium, these cells are recommended for superior growth and protein yields by either baculovirus infection or transfection of appropriate vectors. For cotransfection of plasmids with linearized baculovirus DNA, we recommend Sf9 Insect Cells and BacVector Insect Cell Medium. After recovery from the frozen stock, the cells can be grown as semi-adherent cultures in tissue culture flasks or in suspension as shaker cultures. Cells are shipped on dry ice. Upon receipt, the vials should be removed from their container and either recovered immediately, or placed at -70°C and used within two weeks. For longer-term storage, place in liquid nitrogen.