NovaTaq™ DNA Polymerase

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NovaTaq™ DNA Polymerase

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货号：71003-5

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Description
Overview	NovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl₂ for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl₂, and 25 mM MgCl₂ to enable convenient optimization of Mg²⁺concentration.

Description

Overview

NovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl₂ for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl₂, and 25 mM MgCl₂ to enable convenient optimization of Mg²⁺concentration.

Source	Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration	5.0 U/µl
Purity	> 95% homogeneous by SDS-PAGE
Endonuclease	None detected
Amplification effiency	Functional PCR
Storage	–20°C

Catalogue Number71003 Brand Family Novagen®

References

Product Information
Unit of Definition	One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-<Sup>32</Sup>P]dCTP, and activated salmon sperm DNA.
•	100 U, 500 U, or 5 × 500 U	NovaTaq DNA Polymerase
•	1 or 2 or 7 × 1.5 ml	10X NovaTaq Buffer with MgCl₂
•	1 or 2 or 7 × 1.5 ml	10X NovaTaq Buffer without MgCl₂
•	1 or 2 or 7 × 1.5 ml	25 mM MgCl₂
Declaration	Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

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Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	-20°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

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实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

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NovaTaq™ DNA Polymerase

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