NoShift™ Transcription Factor Assay Kit

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页细胞生物学检测Millipore 密理博>

图片仅供参考，请以实物为准

NoShift™ Transcription Factor Assay Kit

市场价：: 0.00

促销价：: ¥0.00

货号：71377-3

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

重要规格表

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
71377-3CN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知 <a class="null" href="null">联系客户服务</a> Contact Customer Service <a class="null" href="null">联系客户服务</a> <a class="null" href="null">联系客户服务</a>	塑胶安瓿;塑胶针药瓶	96 rxn	正在检索价格...... 无法检索价格。最小订购数量为倍数以订单确认为准更多信息您节约了 ( )。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	The NoShift™ Transcription Factor Assay Kit is a microassay plate–based method for analysis of DNA-binding proteins that alleviates many of the problems associated with gel shift assays. The gel shift, or electrophoretic mobility shift assay (EMSA), is a method for analyzing interactions between proteins and DNA. This technique, first published in 1981 (Fried 1981, Garner 1981), is based on the reduced electrophoretic mobility of protein–DNA complexes compared with that of DNA alone under nondenaturing conditions. The traditional gel shift assay is tedious and time consuming, requires radio-labeled probe, and often requires extensive optimization. The 96-well NoShift plate format is versatile and enables simultaneous analysis of multiple binding factors in less than five hours without the use of radioisotopes. A biotinylated oligonucleotide is mixed with a protein sample and the protein–DNA complex is captured on streptavidin plates. The bound transcription factor is detected with a specific antibody followed by a second antibody–HRP conjugate and chromogenic substrate. Each NoShift Transcription Factor Assay Kit comes with one 96-strip-well streptavidin-coated plate, binding reaction buffer, antibody dilution buffer, plate wash buffer, and TMB substrate and provides enough reagents for 100 reactions. The NoShift Kit is designed for use with oligonucleotides defining a protein binding consensus sequence provided by the purchaser and an antibody to a DNA binding protein.
Catalogue Number	71377
Brand Family	Novagen®

Description

Overview

The NoShift™ Transcription Factor Assay Kit is a microassay plate–based method for analysis of DNA-binding proteins that alleviates many of the problems associated with gel shift assays. The gel shift, or electrophoretic mobility shift assay (EMSA), is a method for analyzing interactions between proteins and DNA. This technique, first published in 1981 (Fried 1981, Garner 1981), is based on the reduced electrophoretic mobility of protein–DNA complexes compared with that of DNA alone under nondenaturing conditions. The traditional gel shift assay is tedious and time consuming, requires radio-labeled probe, and often requires extensive optimization. The 96-well NoShift plate format is versatile and enables simultaneous analysis of multiple binding factors in less than five hours without the use of radioisotopes. A biotinylated oligonucleotide is mixed with a protein sample and the protein–DNA complex is captured on streptavidin plates. The bound transcription factor is detected with a specific antibody followed by a second antibody–HRP conjugate and chromogenic substrate.

Each NoShift Transcription Factor Assay Kit comes with one 96-strip-well streptavidin-coated plate, binding reaction buffer, antibody dilution buffer, plate wash buffer, and TMB substrate and provides enough reagents for 100 reactions. The NoShift Kit is designed for use with oligonucleotides defining a protein binding consensus sequence provided by the purchaser and an antibody to a DNA binding protein.

Catalogue Number

71377

Brand Family

Novagen®

References
References	Fried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.

Product Information
•	4 × 1 ml	4X NoShift™ Bind Buffer
•	150 µl	Salmon Sperm DNA
•	125 µl	Poly(dI:dC):Poly(dI:dC)
•	30 ml	10X NoShift Wash Buffer
•	20 ml	NoShift Antibody Dilution Buffer
•	1 ea	Streptavidin Plate
•	12 ml	TMB Substrate
•	100 µl	100 mM DTT
•	3 ea	Aluminum Plate Sealer

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	Multiple storage requirements
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

帮我找货

购物指南免费注册批量采购购物流程购物保障

配送方式上门自提第三方配送炼石配送

支付方式货到付款账期付款

售后服务售后政策退款说明售后流程免责条款

服务热线

0771-3293894

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

NoShift™ Transcription Factor Assay Kit

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

重要规格表

价格及供货情况

我要咨询