Calcineurin Cellular Activity Assay Kit, Colorimetric

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Calcineurin Cellular Activity Assay Kit, Colorimetric

市场价：: 0.00

促销价：: ¥0.00

货号：207007-1KTT

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity	Detection Methods
H, M, R	Colorimetric

Description
Overview	A colorimetric assay kit for measuring cellular calcineurin (PP2B) enzyme activity. Assays are performed in a convenient 96-well plate format with all reagents necessary for measuring calcineurin activity. The RII Phosphopeptide Substrate (Cat. No. 207008), supplied with this kit, is the most efficient and selective substrate known for calcineurin. The free phosphate released is detected using malachite green.
Catalogue Number	207007
Brand Family	Calbiochem®
Synonyms	PP2B Assay Kit, Colorimetric
Application Data
Materials Required but Not Delivered	• Plate reader capable of measuring A620 to ≥3-decimal accuracy. • Centrifuge capable of 100,000 x g RCF. • Swing bucket centrifuge capable of 800 x g RCF. • Pipettor capable of pipetting 5-100 µl accurately. • Multi-channel pipettor capable of pipetting 100 µl (optional). • Ice bucket to keep reagents cold until use. • 16 gauge needle/syringe. • TBS buffer, 100 ml (150 mM NaCl, 20 mM Tris, pH 7.2) • 15 ml conical centrifuge tubes • Biological test materials (e.g., tissue, cells)

References
References	Mondragon, A., et al. 1997. Biochemistry 36, 4934. Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109. Enz, A., et al. 1994. Anal. Biochem. 216, 147. Harder, K.W., et al. 1994. Biochem. J. 298, 395. Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

Product Information
Detection method	Colorimetric
Form	96 Tests
Format	96-well plate
Kit contains	Human Recombinant Calcineurin as a Positive Control, Bovine Brain Calmodulin, Calcineurin Substrate (Cat. No. 207008), Assay Buffer, EGTA Buffer, Lysis Buffer, Protease Inhibitor Cocktail, Phosphate Detection Agent, Phosphate Standard, Okadaic Acid (Cat. No. 495604), Desalting Column Resin, Chromatography Column, ½ volume 96-Well Plate, and a user protocol.

Applications

Biological Information
Assay time	7 h
Sample Type	Tissue/cellular extracts
Species Reactivity	Human Mouse Rat

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 21/22-36/38 Harmful in contact with skin and if swallowed. Irritating to eyes and skin.
S Phrase	S: 26-24/25 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Avoid contact with skin and eyes.

Product Usage Statements
Intended use	The Calbiochem^® Calcineurin Cellular Activity Assay Kit is a complete colorimetric assay kit for measuring cellular calcineurin (PP-2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin phosphatase activity in tissue/cellular extracts. Human recombinant calcineurin is included as a positive control. The RII phosphopeptide substrate, supplied with this kit is the most efficient and outstanding peptide substrate known for calcineurin. The detection of free phosphate released is based on the malachite green assay and offers the following advantages: non-radioactive, convenient one-step detection, and excellent sensitivity.

Product Usage Statements

Intended use

The Calbiochem^® Calcineurin Cellular Activity Assay Kit is a complete colorimetric assay kit for measuring cellular calcineurin (PP-2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin phosphatase activity in tissue/cellular extracts. Human recombinant calcineurin is included as a positive control. The RII phosphopeptide substrate, supplied with this kit is the most efficient and outstanding peptide substrate known for calcineurin. The detection of free phosphate released is based on the malachite green assay and offers the following advantages: non-radioactive, convenient one-step detection, and excellent sensitivity.

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	Multiple storage requirements
Storage Conditions	Store all components except the plate (room temperature) at -70°C for the highest stability. The calcineurin enzyme component must be handled particularly carefully in order to retain maximal enzymatic activity. Thaw it quickly in a room temperature water bath or by rubbing between your fingers, then immediately store on ice. The remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, aliquot the calcineurin into separate tubes and store at -70°C.
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information
Kit contains	Human Recombinant Calcineurin as a Positive Control, Bovine Brain Calmodulin, Calcineurin Substrate (Cat. No. 207008), Assay Buffer, EGTA Buffer, Lysis Buffer, Protease Inhibitor Cocktail, Phosphate Detection Agent, Phosphate Standard, Okadaic Acid (Cat. No. 495604), Desalting Column Resin, Chromatography Column, ½ volume 96-Well Plate, and a user protocol.