A colorimetric assay kit for measuring calcineurin (PP2B) activity and for inhibitor screening. The assay is performed using RII Phosphopeptide Substrate (Cat. No. 207008).
Catalogue Number
207005
Brand Family
Calbiochem®
Synonyms
PP2B Assay Kit, Colorimetric
Materials Required but Not Delivered
• Plate reader capable of measuring A620 to ≥3-decimal accuracy. • Pipette capable of pipetting 5-100 µl accurately. • Multi-channel pipette capable of pipetting 100 µl (optional). • Ice bucket to keep reagents cold until use.
References
References
Mondragon, A. et al. 1997. Biochemistry36, 4934. Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109. Enz, A., et al. 1994. Anal. Biochem. 216, 147. Harder, K.W., et al. 1994. Biochem. J. 298, 395. Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.
Product Information
Detection method
Colorimetric
Form
96 Tests
Format
96-well plate
Kit contains
Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.
Applications
Biological Information
Assay time
3.5 h
Sample Type
Purified human calcineurin
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 21/22
Harmful in contact with skin and if swallowed.
S Phrase
S: 24/25
Avoid contact with skin and eyes.
Product Usage Statements
Intended use
The Calbiochem® Calcineurin Assay Kit is a complete colorimetric assay kit that is suitable for measuring calcineurin activity. It offers the following advantages: • NON-RADIOACTIVE • CONVENIENT 1-STEP DETECTION - no mixing • 96-WELL-PLATE FORMAT- for high-throughput applications.
Please Note: This kit has not been tested for any other phosphatase activity aside from calcineurin (PP2B) activity and inhibitor screening.
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Storage
≤ -70°C
Storage Conditions
Store all components except the 96-Well Plate (room temperature) at -70°C for the highest stability. In particular, calcineurin must be handled with utmost care in order to retain its maximal enzymatic activity. Thaw it quickly in a water bath, maintained at room temperature, or by rubbing between fingers, then immediately store on an ice bath. Any remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, we recommend to aliquot the calcineurin into separate tubes and then store at -70°C.
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.
新浪微博
腾讯微博
QQ空间
客服
