Overnight Express Autoinduction NMR Medium - 15N, Size = 5 liter kit

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Overnight Express Autoinduction NMR Medium - 15N, Size = 5 liter kit

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促销价：: ¥0.00

货号：71759-4

品牌：Millipore 密理博

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Description
Overview	Overnight Express™ Autoinduction NMR-¹⁵N provides reagents to make a chemically defined autoinducing liquid culture medium used to produce [U-¹⁵N] labeled proteins for NMR spectroscopy. The autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density and to automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG). The system includes four concentrated sterile solutions: OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction; OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis; OnEx Solution 3 provides magnesium for maximal cell density; OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels. The kit also includes ¹⁵N-labeled solid ammonium chloride and reagents to make a non-inducing starter medium, for staged growth without expression and for stable storage of clones. Addition of the four liquid components and a sterile ¹⁵N ammonium chloride solution to sterile water results in a chemically- defined medium promoting high cell densities, autoinduction of expression, and efficient labeling of target proteins. 1 L or 5 L kit sizes are available. The Overnight Express Autoinduction System is covered by US Patents 7,560,264, 7,704,722, and 7,759,109. This Product is sold for research use only. Any commercial use of the Product, including the manufacture of proteins for use in the screening of compound libraries, requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number	71759
Brand Family	Novagen®

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R Phrase	R: 22-36 Harmful if swallowed. Irritating to eyes.
S Phrase	S: 36 Wear suitable protective clothing.

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Ship Code	Multiple Storage Conditions
Toxicity	Harmful
Storage	Multiple storage requirements
Do not freeze	Ok to freeze

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Overnight Express Autoinduction NMR Medium - 15N, Size = 5 liter kit

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