Overnight Express™ autoinduction media are based on technology developed by F. William Studier at Brookhaven National Laboratory and enable regulated protein expression in E. coli, without monitoring the culture or adding IPTG during cell growth. The method uses media components that are metabolized differentially to promote growth to high cell density and automatically induce protein expression from lac promoters. As a result, these unique autoinduction media provide high-level protein production in pET and other IPTG-inducible bacterial expression systems. Cell mass and target protein yield are often increased several-fold compared to conventional protocols using induction with IPTG. Autoinduction media are extremely convenient for routine expression of proteins in multiple cultures and are ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones.


The Overnight Express Autoinduction System is covered by US Patents 7,560,264, 7,704,722, and 7,759,109. This Product is sold for research use only. Any commercial use of the Product, including the manufacture of proteins for use in the screening of compound libraries, requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.