Glutathione Assay Kit II

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Glutathione Assay Kit II

市场价：: 0.00

促销价：: ¥0.00

货号：354103-1KIT

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Detection Methods
Colorimetric

Description
Overview	A sensitive and convenient colorimetric (414 or 405 nm) assay kit for the measurement of glutathione from plasma, serum, erythrocyte lysate, tissue samples, and cultured cells. Dynamic range: 0 - 16 µM for GSH or 0 - 8 µM for GSSG. For plasma and serum samples concentration and deproteination are required.
Catalogue Number	354103
Brand Family	Calbiochem®
Application Data
Materials Required but Not Delivered	• A plate reader with a 414 or 405 nm filter. • An adjustable pipettor, repeat pipettor, and an eight-channel pipettor (optional). • A source of pure water. Glass distilled water or HPLC-grade water is acceptable. • Metaphosphoric acid and triethanolamine (described in Recommended procedure for deproteination of samples). • 2-vinylpyridine (optional, described in Sample preparation for exclusive measurement of GSSG).

References
References	Foyer, C.H., et al. 1994. Physiol. Plant. 92, 696. Baillie, T.A. and Slatter, J.G. 1991. Acc. Chem. Res. 24, 264. Baker, M.A., et al. 1990. Anal. Biochem. 190, 360. Inoue, M., et al. 1987. Journal of Protein Chemistry 6, 207. Eyer, P. and Podhradsky, D. 1986. Anal. Biochem. 153, 57. Inoue, M. 1985. in Renal Biochemistry. Kinne, R.K.H. editor. Elsevier Science Publishers B.V. London, 225. Lash, L.H. and Jones, D.P. 1985. Arch. Biochem. Biophys. 240, 583. Griffith, O.W. 1980. Anal. Biochem. 106, 207. Wendel, A. and Cikryt, P. 1980. FEBS Lett. 120, 209. Glutathione: Metabolism and function, Arias, I.M. and Jakoby, W.B. 1976. editors. Raven Press, New York. Tietze, F. 1969. Anal. Biochem. 27, 502.

References

Foyer, C.H., et al. 1994. Physiol. Plant. 92, 696.
Baillie, T.A. and Slatter, J.G. 1991. Acc. Chem. Res. 24, 264.
Baker, M.A., et al. 1990. Anal. Biochem. 190, 360.
Inoue, M., et al. 1987. Journal of Protein Chemistry 6, 207.
Eyer, P. and Podhradsky, D. 1986. Anal. Biochem. 153, 57.
Inoue, M. 1985. in Renal Biochemistry. Kinne, R.K.H. editor. Elsevier Science Publishers B.V. London, 225.
Lash, L.H. and Jones, D.P. 1985. Arch. Biochem. Biophys. 240, 583.
Griffith, O.W. 1980. Anal. Biochem. 106, 207.
Wendel, A. and Cikryt, P. 1980. FEBS Lett. 120, 209.
Glutathione: Metabolism and function, Arias, I.M. and Jakoby, W.B. 1976. editors. Raven Press, New York.
Tietze, F. 1969. Anal. Biochem. 27, 502.

Product Information
Detection method	Colorimetric
Form	96 Tests
Format	96-well plate
Kit contains	2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.
Positive control	GSSG

Applications

Biological Information
Assay range	0 - 16 µM for GSH or 0 - 8 µM for GSSG
Assay time	3 h
Sample Type	Plasma, serum, erythrocyte lysate, tissue samples, and cultured cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Storage Conditions	Upon arrival store entire kit contents at 4°C.
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information
Kit contains	2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.