A spectrophotometric assay kit that is specific for reduced glutathione (GSH) or total mercaptans. Absorbance is read at ~400 nm for GSH and ~356 nm for total mercaptens.
Note: 1 T = 1 test.
Catalogue Number
354102
Brand Family
Calbiochem®
Materials Required but Not Delivered
• Glutathione, Reduced (GSH) (Cat. No. 3541), purity >98% • Metaphosphoric acid (Cannot be substituted by any other acid), purity 33-37% • Spectrophotometer with absorbance measurable at 356 and 400 nm (from 0 to 2 absorbance unit). • Spectrophotometric cuvettes with 1-ml volume and 1-cm path length. • Cuvettes made of glass or plastic are suitable.
References
References
Ji, L.L., and Fu, R. 1992. J. Appl. Physiol.72, 549. Anderson M.E. 1989. Enzymatic and chemical methods for the determination of Glutathione; In: Glutathione: chemical, biochemical and medical aspects, Vol. A, Dolphin D., Poulson R. and Avramovic O. Eds., John Wiley and Sons, pp. 339-365. Dolphin D., et al. 1989. Glutathione: Chemical, Biochemical and Medical Aspects, Vols A & B, J. Wiley and Sons.
Product Information
Detection method
Colorimetric
Form
100 Tests
Format
Cuvette
Kit contains
A Chromogen, 30% NaOH, Buffer, and a user protocol.
Applications
Biological Information
Assay time
1.5 h
Sample Type
Cell lysates, tissue homogenates
Physicochemical Information
Sensitivity
5.0 µM RSH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 34
Causes burns.
S Phrase
S: 23-26-27-36/37/39
Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Take off immediately all contaminated clothing. Wear suitable protective clothing, gloves and eye/face protection.
Product Usage Statements
Intended use
The Calbiochem® Glutathione Assay Kit makes it possible to assay for glutathione with only one sampling and one colorimetric measurement. A modification of this method makes it possible to assay other mercaptans. This alternate protocol is based on the measurement of substitution products that absorb maximally at 356 nm in the absence of reagent R2. Due to its ease of use, this method is well adapted for the quantification of glutathione and total mercaptans in a large number of biological samples. The main advantage of the method is that it does not require any enzyme as a reagent.
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+2°C to +8°C
Storage Conditions
Upon arrival store the entire kit contents at 4°C. • All solutions of reagents and buffer have been tightly sealed and are stable if properly stored between 4°C. Do Not Freeze. Protect from light. • After removing the required amount of each reagent for immediate use, all bottles should be tightly closed and stored between 2-4°C. Do not leave the reagent bottles open or at room temperature.
Protect from Light
Protect from light
Do not freeze
Yes
Packaging Information
Transport Information
Supplemental Information
Kit contains
A Chromogen, 30% NaOH, Buffer, and a user protocol.
新浪微博
腾讯微博
QQ空间
客服
