EZ-Nuclear RIP (Native) Kit

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页细胞生物学检测Millipore 密理博>

图片仅供参考，请以实物为准

EZ-Nuclear RIP (Native) Kit

市场价：: 0.00

促销价：: ¥0.00

货号：17-10523

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

特卖

Magna Grip™ Rack 8 well (Cat.# 20-400) Now Available! or similar magnetic rack.

重要规格表

Key Applications
RIP, Protein Interaction Assays

Description
Catalogue Number	17-10523
Trade Name	Magna Nuclear RIP
Description	EZ-Magna Nuclear RIP™ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit
Overview	Features and Advantages Generates native chromatin to allow analysis of a variety of chromatin-associated RNAs Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 100,000 cells (native) Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios Suitable for analysis by RT-qPCR or RIP-seq Complete set of reagents and detailed protocol to enable first-time success Includes positive and negative control antibodies and qPCR primers Magna Nuclear RIP™ Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq). Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. In contrast, nuclear RIP with cross-linked chromatin can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. For less well understood proteins and protein complexes often both approaches are used. The kit described here is for the native approach. If a cross-linked approach is of interest please visit the product page for the Magna Nuclear RIP (Cross-Linked) Kit , catalogue # 17-10520 or the EZ-Magna Nuclear RIP (Cross-Linked) Kit, catalogue # 17-10521.
Materials Required but Not Delivered	Reagents Cells or tissue, stimulated or treated as needed for the experimental system Antibody of interest for RNA-binding protein immunoprecipitation (RIP) Negative Control Antibody PBS (RNase free) (e.g. Fisher, Cat. # BP2438-4) TRIzol® Reagent Life Technologies Cat. #15596-026) or similar organic extraction reagent Chloroform (e.g. Fisher, Cat. # BP1145) Isopropanol (molecular biology grade) 100% Ethanol (molecular biology grade) Precipitation Carrier (e.g. Pellet Paint® Co-Precipitant (125 reactions, EMD Millipore Cat. # 69049) or linear Acrylamide (5mg/mL) if samples are intended for Next Gen Sequence library preparation. Nuclease Free Water Liquid nitrogen (Optional) Reagents for qRT-PCR Analysis and/or Reagents for RNA-Seq Library Construction (optional) Equipment 1 mL Syringes 27G Needles Dounce homogenizer (loose pestle, necessary for tissue samples but optional for cultured cells) Magnetic Separation Stand (e.g. Magna GrIP™ Rack 8 well, (Cat. # 20-400) or PureProteome™ Magnetic Stand, (Cat. # LSKMAGS08) Vortex mixer Rotating wheel/platform Microcentrifuge capable of up to 12,000 x g -80°C freezer Variable temperature water bath or incubator Rotating microtube mixer Real-time PCR thermal cycler

References

Product Information
Components	Nuclei Isolation Buffer RIP Lysis Buffer Protein A/G Magnetic Beads RIP Lysis Buffer 0.5M EDTA DNase I (RNase Free) 2 U/μL DNase I Reaction Buffer Protease Inhibitor Cocktail III, Animal Free RNAse Inhibitor Control Antibodies and Primers Normal Mouse IgG Negative Control Antibody Anti-EZH2 Positive Control Antibody NEAT1 Positive Control Primers U1 snRNA Negative Control Primers
Presentation	Two boxes containing key reagents for generation of native nuclear lysates and performance of 24 individual RNA-binding protein immunoprecipitation (RIP) reactions. Plus box containing positive and negative control antibodies and qPCR primers.

Applications
Application	EZ-Magna Nuclear RIP (Native) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.
Key Applications	RNA Binding Protein Immunoprecipitation (RIP) Protein Interaction Assays

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.

Packaging Information
Material Size	24 assays
Material Package	Kit capacity: 24 RNA-binding protein immunoprecipitation assays using a native (non-cross-linked) nuclear lysate