In Vitro Tubulin Polymerization Assay Kit (>99% Pure Bovine Tubulin)

Description
Catalogue Number	17-10194
Brand Family	Chemicon®
Trade Name	Chemicon
Description	In Vitro Tubulin Polymerization Assay Kit (≥99% Pure Bovine Tubulin)
Overview	Introduction Microtubules are long, hollow cylinders made up of polymerised α- and β-tubulin dimers. Tubulin dimers polymerize end to end in protofilaments which are the building block for the microtubule structure. Microtubules are part of a structural network (the cytoskeleton) within the cell's cytoplasm. The primary role of the microtubule cytoskeleton is mechanical. However, in addition to structural support, microtubules also take part in many other processes. A microtubule is capable of growing and shrinking in order to generate force, and there are also motor proteins that allow organelles and other cellular factors to be carried along a microtubule. Tubulin is a highly-conserved major component of eukaryotic cells and the ‘building block’ of microtubules. Tubulin is a heterodimer of α and β subunits with an apparent molecular mass of ~110 kDa. In vivo and in vitro, in the presence of GTP, tubulin heterodimers can polymerize (assemble) to form microtubules. Many proteins or small-molecule compounds affect microtubule assembly. EMD Millipore’s In vitro Tubulin Polymerization Assay Kit allows for quick and quantitative determination of tubulin polymerization due to protein and compound-based modulation.
Alternate Names	tubulin polymerization bovine tubulin
Materials Required but Not Delivered	• Distilled water • 96-well plate spectrophotometer able to read optical densities at 350 nm with temperature control (a must) and shaking (optional). • Bench top Ultracentrifuge Beckman Optima or equivalent (optional). • Pipettes, Micropipettes and Tips. • Microcentrifuge Tubes.

Description

Catalogue Number

17-10194

Brand Family

Chemicon®

Trade Name

Chemicon

Description

In Vitro Tubulin Polymerization Assay Kit (≥99% Pure Bovine Tubulin)

Overview

Introduction
Microtubules are long, hollow cylinders made up of polymerised α- and β-tubulin dimers. Tubulin dimers polymerize end to end in protofilaments which are the building block for the microtubule structure. Microtubules are part of a structural network (the cytoskeleton) within the cell's cytoplasm. The primary role of the microtubule cytoskeleton is mechanical. However, in addition to structural support, microtubules also take part in many other processes. A microtubule is capable of growing and shrinking in order to generate force, and there are also motor proteins that allow organelles and other cellular factors to be carried along a microtubule.

Tubulin is a highly-conserved major component of eukaryotic cells and the ‘building block’ of microtubules. Tubulin is a heterodimer of α and β subunits with an apparent molecular mass of ~110 kDa. In vivo and in vitro, in the presence of GTP, tubulin heterodimers can polymerize (assemble) to form microtubules.

Many proteins or small-molecule compounds affect microtubule assembly. EMD Millipore’s In vitro Tubulin Polymerization Assay Kit allows for quick and quantitative determination of tubulin polymerization due to protein and compound-based modulation.

Alternate Names

tubulin polymerization
bovine tubulin

Materials Required but Not Delivered

• Distilled water
• 96-well plate spectrophotometer able to read optical densities at 350 nm with temperature control (a must) and shaking (optional).
• Bench top Ultracentrifuge Beckman Optima or equivalent (optional).
• Pipettes, Micropipettes and Tips.
• Microcentrifuge Tubes.

Product Information
Components	≥99% Pure Bovine Tubulin: 2 vials of 130 µL (240 µM) each of ≥99% pure bovine tubulin stock solution in 1X polymerization buffer (PB) containing 1mM GTP. Polymerization Buffer (PB) 5X: One vial containing 300 µL 5X polymerization buffer. 200mM GTP: 1 vial containing 10 µL of 200 mM GTP. One 96-well half area plate. Paclitaxel: One vial containing 10 µL of 700 µM paclitaxel in DMSO. Nocodazole: One vial containing 10 µL of 700 µM nocodazole in DMSO.

Product Information

Components

≥99% Pure Bovine Tubulin: 2 vials of 130 µL (240 µM) each of ≥99% pure bovine tubulin stock solution in 1X polymerization buffer (PB) containing 1mM GTP.
Polymerization Buffer (PB) 5X: One vial containing 300 µL 5X polymerization buffer.
200mM GTP: 1 vial containing 10 µL of 200 mM GTP.
One 96-well half area plate.
Paclitaxel: One vial containing 10 µL of 700 µM paclitaxel in DMSO.
Nocodazole: One vial containing 10 µL of 700 µM nocodazole in DMSO.

Applications
Application	This In vitro Tubulin Polymerization Assay Kit used >99% pure bovine tubulin to allow for quick and quantitative determination of tubulin polymerization.
Application Notes	EMD Millipore’s In vitro Tubulin Polymerization Assay Kit allows for quick and quantitative determination of tubulin polymerization due to protein and compound-based modulation.

Applications

Application

This In vitro Tubulin Polymerization Assay Kit used >99% pure bovine tubulin to allow for quick and quantitative determination of tubulin polymerization.

Application Notes

EMD Millipore’s In vitro Tubulin Polymerization Assay Kit allows for quick and quantitative determination of tubulin polymerization due to protein and compound-based modulation.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store tubulin stock solution at -80°C, store all other components at -20°C for up to 4 months from date of receipt. Do not freeze and thaw.

Storage and Shipping Information

Storage Conditions

Store tubulin stock solution at -80°C, store all other components at -20°C for up to 4 months from date of receipt. Do not freeze and thaw.

Packaging Information
Material Size	16 assays

Packaging Information

Material Size

16 assays

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

In Vitro Tubulin Polymerization Assay Kit (>99% Pure Bovine Tubulin)

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