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Ati-GPR30, clone 2F2 (mouse monoclonal)

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货号：MABS279

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IHC, IP M Purified Monoclonal Antibody
Description
Catalogue Number MABS279
Description Anti-GPR30, clone 2F2 Antibody
Alternate Names
  • G-protein coupled estrogen receptor 1
  • Chemoattractant receptor-like 2
  • Flow-induced endothelial G-protein coupled receptor 1
  • FEG-1
  • G-protein coupled receptor 30
  • GPCR-BR
  • IL8-related receptor DRY12
  • Lymphocyte-derived G-protein coupled receptor
  • LYGPR
  • Membrane estrogen receptor
  • mER
Background Information GPR30, also known as G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1 (FEG-1), G-protein coupled receptor 30, GPCR-BR, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor (LYGPR), or Membrane estrogen receptor (mER), and encoded by the gene GPER/CEPR/CMKRL2/DRY12/GPR30,is the common receptor for estrogen. GPR30 binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol (3,4,5)-trisphosphate in the nucleus, and therefore GPR30 is part of the rapid non-genomic signaling events widely observed when cells interact with estrogen. GPR30 is a cell membrane bound protein found in the Golgi apparatus, endoplasmic reticulum and plasma membrane. GPR30 is ubiquitously expressed, but is most abundant in placenta. In brain regions, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Detect GPR30 using this Anti-GPR30 antibody, clone 2F2 validated for use in IHC & IP.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes Immunoprecipitation Analysis: A representative lot from an independent laboratory detected GPR30 in HEK293 cell lysate (Filardo, E., et al. (2007). Endocrinology. 148(7):3236-3245.).
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to the C-terminus of human GPR30.
Epitope C-terminus
Clone 2F2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GPER
  • CEPR
  • CMKRL2
  • DRY12
  • GPR30
Purification Method Protein G Purified
UniProt Number
Molecular Weight 42 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Immunohistochemistry in human placenta tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GPR30 in human placenta tissues.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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