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| Replacement Information |
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| Description | |
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| Catalogue Number | 17-10451 |
| Replaces | Replaces S7820 (CpGenome Universal DNA Modification Kit). |
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| Description | CpGenome Direct Prep Bisulfite Modification Kit (50 Reactions) |
| Overview | Unlike other bisulfite modifications approaches that start with isolated genomic DNA, the CpGenome Direct Prep Bisulfite Modification Kit allows simple and reliable bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue and fixed tissue samples. Key Features
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| Materials Required but Not Delivered | • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602) |
| Background Information | Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation. |
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| Applications | |
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| Application | The CpGenome Direct Prep Bisulfite Modification Kit allows bisulfite conversion directly from a variety of starting materials, including cultured cells, blood, fresh tissue & fixed tissue samples. |
| Application Notes | For MSP primer design, please use the MethPrime software package. Click here |
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| Material Size | 50 reactions |
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